摘要
为了快速、高效地提取活性污泥的胞外聚合物,对比分析了超声法、表面活性剂法、表面活性剂增强超声法的提取效果,并确定了最优提取条件。研究发现,表面活性剂增强超声法的提取量最高。最优提取条件:超声功率为100 W,超声时间为5 min,CTAB投加量为48 mg。3种方法主要的红外吸收峰均与蛋白质、多糖等物质的官能团密切相关,且表面活性剂增强超声法所提取的EPS的荧光强度最高峰的种类最全面,表明表面活性剂增强超声法是活性污泥EPS的最佳提取方法。
In order to extract extracellular polymers from activated sludge rapidly and efficiently,the extraction effects of ultrasonic method,surfactant method and surfactant-enhanced ultrasonic method were compared and analyzed,and the optimal extraction conditions were determined.The results showed that the extraction content of surfactant-enhanced ultrasonic method was the highest.The optimal extraction conditions are conditions of 100 W ultrasonic power,5 min ultrasonic action time and 48 mg CATB dosage.The main infrared absorption peaks of all three methods were closely related to the functional groups of proteins,polysaccharides,and the kinds of the highest fluorescence intensity peaks of EPS extracted by surfactant-enhanced ultrasonic method were the most comprehensive,indicating this method was the best extraction method for extracting EPS from activated sludge.
作者
周语桐
杨英
李卫华
张田田
吴亮
程志龙
ZHOU Yutong;YANG Ying;LI Weihua;ZHANG Tiantian;WU Liang;CHENG Zhilong(School of Environmental and Energy Engineering,Anhui Jianzhu University,Hefei Anhui 230601;Anhui Provincial Key Laboratory of Water Pollution Control and Wastewater Recycling,Anhui Jianzhu University,Hefei Anhui 230601)
出处
《湖北理工学院学报》
2023年第1期55-59,共5页
Journal of Hubei Polytechnic University
基金
国家自然科学基金项目(项目编号:21677001)
安徽省科技重大专项项目(项目编号:201903a06020034,17030801028)
省级“六卓越一拔尖”卓越人才培养创新项目(项目编号:2019zyrc085)
环境生态工程卓越工程师教育培养计划项目(项目编号:2019zyrco85)。
关键词
活性污泥
胞外聚合物
表面活性剂
超声波
activated sludge
extracellular polymer
surface active agent
ultrasonic