MiR⁃129⁃5p在肺腺癌中的表达及其临床意义

Expression of MiR⁃129⁃5p in lung adenocarcinoma and its clinical significance

目的探讨肺腺癌组织中微小RNA⁃129⁃5p(MiR⁃129⁃5p)的表达变化及临床意义。方法选取2014年1月至2017年1月本院收治的50例肺腺癌患者(肺腺癌组),和30名健康者(健康对照组)。所有患者血清中提取RNA。使用QiagenValenicaCA将RNA逆转录为cDNA。使用QSystem(Qiagen)进行定量PCR(qPCR)。分析MiR⁃129⁃5p表达变化与肺腺癌患者临床病理特征的关系;对患者随访5年,分析MiR⁃129⁃5p表达变化与患者生存预后的关系。建立受试者操作特征(ROC)曲线,对微小RNA⁃129⁃5p诊断肺腺癌效率进行评估。结果与健康对照组比较,肺腺癌血清中MiR⁃129⁃5p相对表达量低,差异有统计学意义(t=48.215,P<0.001)。MiR⁃129⁃5p表达与肺腺癌分化程度、淋巴结转移、远处转移、年龄(>60岁与<60岁)、TNM分期、浸润深度、表皮生长因子受体(EGFR)基因突变有关(P均<0.05),与患者性别、吸烟与否、肿瘤大小无关(P>0.05)。以肺腺癌血清中MiR⁃129⁃5p表达的中位数为基准,将肺腺癌分为高、低表达组。MiR⁃129⁃5p高表达组5年总生存率高于MiR⁃129⁃5p低表达组,差异有统计学意义(χ^(2)=23.765,P<0.05)。通过绘制ROC曲线MiR⁃129⁃5p诊断肺腺癌的敏感性为65.6%,特异性为75.0%,AUC为0.751(P<0.05),临界值0.452,95%CI为0.613~0.887。结论肺腺癌组织中MiR⁃129⁃5p低表达,其表达变化与肺腺癌发生发展及预后有关;血清MiR⁃129⁃5p可用作肺腺癌癌症诊断和预后的潜在生物标志物。

Objective To investigate the expression of microrna⁃129⁃5p(MiR⁃129⁃5p)in lung adenocarcinoma and its clinical significance.Methods 80 patients treated in our hospital from January 2014 to January 2017 were divided into 30 healthy control subjects and 50 patients with lung adenocarcinoma.RNA was extracted from the sera of all subjects.RNA was reverse transcribed into cDNA using Qiagenvalenicaca.Quantitative PCR(qPCR)was performed using Qsystem(Qiagen).The relationship between the expression changes of MiR⁃129⁃5p and the clinicopathological features of patients with lung adenocarcinoma was analyzed;the patients were followed up for 5 years,and the relationship between the changes of MiR⁃129⁃5p expression and the survival and prognosis of patients was analyzed.Receiver operating characteristic(ROC)curve was established to evaluate the efficiency of microRNA⁃129⁃5p in the diagnosis of lung adenocarcinoma.Results Compared with the healthy control group,the relative expression of MiR⁃129⁃5p in serum of lung adenocarcinoma was low(t=48.215,P<0.001).The expression of MiR⁃129⁃5p was related to the degree of differentiation,lymph node metastasis,distant metastasis,age(>60 years and<60 years),TNM stage,depth of invasion and epidermal growth factor receptor(EGFR)gene mutation of lung adenocarcinoma(P<0.05).It was not related to gender,smoking and tumor size(P>0.05).Based on the median expression of MiR⁃129⁃5p in serum of lung adenocarcinoma,lung adenocarcinoma was divided into high and low expression groups.The 5⁃year overall survival rate of the MiR⁃129⁃5p high expression group was higher than that of the MiR⁃129⁃5p low expression group(χ^(2)=23.765,P<0.05).By plotting ROC curve,the sensitivity of MiR⁃129⁃5p in the diagnosis of lung adenocarcinoma was 65.6%,the specificity was 75.0%,AUC=0.751,P=0.002<0.05,the critical value was 0.452,and 95%CI:0.613~0.887.Conclusion The expression of MiR⁃129⁃5p was low in lung adenocarcinoma,and its expression changes were related to the occurrence,development and prognosis of lung adenocarcinoma;Serum MiR⁃129⁃5p can be used as a potential biomarker for the diagnosis and prognosis of lung adenocarcinoma.