稻虾鳝立体综合种养水体微生物多样性分析

Study on Microbial Diversity of Water in Rice-crayfish-eel Coculture System

为探讨稻虾鳝立体综合种养水体微生物群落结构,在稻虾综合种养水体不同区域设置网箱养殖黄鳝。运用Illumina MiSeq测序技术,对系统中网箱内(T1)、网箱外(T2)、邻侧环沟(T3)、对侧环沟(T4)4个采样点水体的微生物多样性进行比较。结果表明,稻虾鳝立体综合种养系统水体16S rRNA基因集成3737个操作分类单元(OTU),共鉴定出47门、137纲、309目、509科、929属、1697种,变形菌门、蓝细菌门和放线菌门为采样点共同的优势菌门。网箱养殖黄鳝未对水体不同区域微生物多样性指数造成显著影响,但显著改变了微生物群落结构。随着养殖的进行,不同采样点间菌落组成差异变大。冗余分析(RDA)和直系同源基因簇(COG)功能预测结果显示,亚硝酸盐氮(NO_(2)^(-)-N)、总氮(TN)和氨态氮(NH_(4)^(+)-N)含量与微生物群落呈现出较强的相关性,高锰酸盐指数(COD_(Mn))与微生物群落相关性最小,系统内与氨基酸代谢和能量转换相关的菌群丰度最高。

To study the microbial diversity in different spatial regions of rice-crayfish-eel coculture system,Illumina MiSeq was used to analyze the microbial diversity of water samples inside the cages(T1),outside the cages(T2)and different positions in circular groove(T3,T4).The results showed that a total of 3737 OTUs were obtained from 16S rRNA gene in water samples from the system of rice-crayfish-eel,and a total of 47 phyla,137 classes,309 orders,509 families,929 genera and 1697species were identified.Proteobacteria,Cyanobacteria and Actinobacteria were the dominant groups in different sampling sites.Eel cage-culture did not significantly affect the microbial diversity index in different areas of water,but significantly changed the microbial community structure.With the development of culture,the difference of bacterial composition among different sampling sites was more and more obvious.Redundancy analysis(RDA)results showed that NO_(2)^(-)-N,TN and NH_(4)^(+)-N were highly correlated with the microbial community composition of the system,and COD_(Mn)had the least correlation with the microbial community composition.Cluster of orthologous group(COG)functional prediction analysis showed that the bacteria associated with amino acid metabolism and energy conversion had the highest abundance.