贵州废弃烟叶抗炎活性部位及其化学成分分析鉴定

Analysis of Chemical Components and Anti-inflammatory Activity Parts of Guizhou Waste Tobacco Leaves

以贵州废弃烟叶为原料,通过提取、浓缩和柱层析等方法制备得到废弃烟叶乙醇提取物中不同极性的粗分段部分。采用NO试剂盒检测不同极性部分对RAW 264.7炎症细胞生成NO的影响,研究发现其乙酸乙酯部分和正丁醇部分的抗炎活性尤为突出。通过高分辨质谱Q Exactive Focus分析鉴定乙酸乙酯和正丁醇抗炎活性部分中的化学成分,结果表明两个活性部分中部分化合物具有抗炎活性作用,为进一步探讨多用途废弃烟叶和研制出天然抗炎产品提供一定的研究基础。

Using Guizhou waste tobacco leaves as raw materials,the crude segmented parts of ethanol extract of waste tobacco leaves with different polarity were prepared by extraction,concentration and column chromatography.The effects of different polar parts on NO production by inflammatory cells of RAW 264.7 were detected by NO kit,and it was found that the anti-inflammatory activities of ethyl acetate fraction and n-butanol fraction were particularly prominent.The chemical components in the anti-inflammatory active fractions of ethyl acetate and n-butanol were analyzed and identified by high-resolution mass spectrometry Q Exactive Focus.The results showed that some compounds in the two active fractions had anti-inflammatory activity,which provided a certain research basis for further exploring multipurpose waste tobacco leaves and developing natural anti-inflammatory products.