赶黄草醇提物和赶黄草总黄酮对小鼠亚急性酒精性肝损伤保护作用的比较研究

Comparative Study on Hepatoprotective Effects of Alcohol Extract and Total Flavonoids of Penthorum chinense Pursh against Alcohol-induced Subacute Liver Injury in Mice

目的:比较赶黄草醇提物和赶黄草总黄酮对小鼠亚急性酒精性肝损伤的保护作用。方法:将昆明小鼠随机分为正常组、模型组、阳性组(联苯双酯,150 mg/kg)、赶黄草醇提物3个不同剂量处理组(400、800和1600 mg/kg)及赶黄草总黄酮3个不同剂量处理组(100、200和400 mg/kg);应用酒精灌胃方法建立亚急性酒精性肝损伤小鼠模型,检测各组小鼠肝脏系数、肝组织病理变化和血清生化指标。结果:与模型组比较,赶黄草醇提物和赶黄草总黄酮高、中剂量组小鼠肝脏系数均显著降低(P<0.05),肝组织损伤和脂肪变性明显减轻,小鼠血清谷丙转氨酶(alanine transaminase,ALT)、谷草转氨酶(aspartate transaminase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)、乳酸脱氢酶(lactic dehydrogenase,LDH)活性及总胆红素(total bilirubin,TBIL)、总胆固醇(total cholesterol,TC)、甘油三酯(triglycerides,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)含量均显著降低(P<0.05,P<0.01)。与等生药剂量的赶黄草醇提物组比较,赶黄草总黄酮组小鼠血清ALT、AST、ALP、LDH活性及TBIL、TC、TG和LDL-C含量均显著降低(P<0.05,P<0.01)。结论:赶黄草醇提物和赶黄草总黄酮对亚急性酒精性肝损伤均具有保护作用,赶黄草总黄酮优于赶黄草醇提物,赶黄草总黄酮是赶黄草抗酒精性肝损伤的主要功效成分。

Object:To compare the hepatoprotective effects of alcohol extract and total flavonoids of Penthorum chinense Pursh on alcohol-induced subacute liver injury in mice.Methods:The mice were randomized into the normal group,model group,positive group(bifendate,150 mg/kg),ethanol extract of Penthorum chinense Pursh with three different dose treatment groups(400,800 and 1600 mg/kg),and total flavonoids of Penthorum chinense Pursh with three different dose treatment groups(100,200 and 400 mg/kg).The model of subacute alcoholic liver injury in mouse was set up by intragastric administration of alcohol.The liver index,pathological changes of liver tissue and serum biochemical indexes were measured in each group.Results:Compared with mice in the model group,the hepatic index was decreased significantly(P<0.05),the liver injury pathological state was ameliorated,the activities of serum alanine transaminase(ALT),aspartate transaminase(AST),alkaline phosphatase(ALP),lactic dehydrogenase(LDH)and the content of total bilirubin(TBIL),total cholesterol(TC),triglycerides(TG),low density lipoprotein cholesterol(LDL-C)were decreased significantly(P<0.05,P<0.01)in the high and medium treatment groups of alcohol extract and total flavonoids of Penthorum chinense Pursh.The activities of serum ALT,AST,ALP,LDH as well as the content of TBIL,TC,TG and LDL-C in the total flavonoids group were significantly lower than those in the alcohol extract group with the same crude drug dose(P<0.05,P<0.01).Conclusion:Both alcohol extract and total flavonoids of Penthorum chinense Pursh can protect subacute alcoholic liver injury,but total flavonoids does better than alcohol extract.The total flavonoids is a main functional component of Penthorum chinese Pursh to protect alcoholic liver injury.