电化学分析法检测抗坏血酸、多巴胺和尿酸研究进展

Research progress in electrochemical detection of ascorbic acid,dopamine and uric acid

抗坏血酸(ascorbic acid,AA)、多巴胺(dopamine,DA)和尿酸(uric acid,UA)分别作为抗氧化剂、神经递质和蛋白质代谢物共存于人体内。体内AA、DA和UA水平异常可引发多种疾病,定量检测其水平对相关疾病的预防和诊断具有重要意义。电化学分析法具有成本低、响应快速、使用方便、灵敏度高、选择性好等突出优点,广泛用于生物活性分子的检测。AA、DA和UA具有高电化学活性,适合采用电化学分析法进行测定。然而,它们的氧化峰电位非常相近,在电化学检测中容易产生重叠峰,从而干扰检测结果。针对这一问题,国内外学者通过发展不同的电极材料以提升检测的选择性和灵敏度。本综述总结了近年来电化学单独或同时检测UA、DA和AA的研究进展。

Ascorbic acid(AA),dopamine(DA)and uric acid(UA)coexist in the human body as antioxidants,neurotransmitters and protein metabolites,respectively.Abnormal levels of AA,DA and UA may cause many diseases.Quantitative detection of their levels is important in the prevention and,,,diagnosis of related diseases.Electrochemical detection has low cost fast response convenient use good sensitivity and selectivity,which is widely used in the detection of bioactive molecules.AA,DA and UA have high electrochemical activity,and are suitable for electrochemical analysis.However,,their oxidation peak potentials are very similar so overlapping peaks are easily generated in the electrochemical detection,thus interfering the detection results.To solve this problem,domestic and foreign scholars have developed different electrode materials to improve the selectivity and sensitivity.This review summarized recent advances in the individual or simultaneous determination of UA,DA and AA by electrochemistry.