γ-聚谷氨酸—壳聚糖复合材料的制备及其止血和促创面愈合性能研究

Research on the Preparation ofγ-Polyglutamic Acid-Chitosan Composite Materials for Hemostasis and Wound-Healing Applications

以γ-聚谷氨酸和壳聚糖为原料制备新型复合止血材料,γ-聚谷氨酸在钙离子的作用下交联生成聚谷氨酸钙,进一步与壳聚糖凝胶化反应制备止血材料,采用红外光谱、扫描电镜、吸水性测试、体外凝血测试、溶血率实验、细胞毒性实验测试其性能。当止血材料中壳聚糖和聚谷氨酸钙的比例为10∶1时,材料为层状堆积结构,具有较高的比表面积和较多孔隙,此时材料的吸水率为813.0%,凝血指数为18.97%,样品质量浓度为10mg/mL时的溶血率为4.63%,对L929细胞的增值无明显影响。体内止血实验结果显示,该材料可在30s内快速止血,无二次出血现象,且对创面愈合具有一定的促进作用。上述结果表明,该材料具有良好的体外止血性能,且效果优于市售止血产品Celox,应用前景广阔。

This paper focuses on the preparation of novel chitosan-polyglutamic acid calcium salt composite materials and their hemostatic properties investigation in vivo and in vitro.Crosslinking of polyglutamic acid in the presence of calcium ion afforded polyglutamic acid calcium salt,the process was followed by gelation with chitosan to provide the desired materials.The properties of the materials were investigated by Fourier transform infrared spectroscopy,scanning electron microscope,water absorption test,blood clotting index study,hemolytic test,and cytotoxicity assay.The materials showed layered structure when the mass ratio of chitosan and polyglutamic acid calcium salt was 10:1,which indicated that the material might own high specific surface area and various porosities.The water absorption ratio of the materials was 813.0%,and the blood clotting index was as low as 18.97%.The hemolysis ratio was determined to be 4.63%when the concentration of the materials was 10 mg/mL,and the material showed no obvious effect on the proliferation of L929 cells.Furthermore,the material could stop bleeding in 30 seconds without secondary hemorrhage and obviously promote the wound-healing process.Compared with the hemostatic material Celox available on the market,the synthetic materials exhibited good hemostatic properties and application prospects.