胎盘ABCB4表达在预测妊娠期肝内胆汁淤积症孕妇妊娠结局中的意义

Significance of Placental ABCB4 Expression in Predicting Pregnancy Outcome in Pregnant Women with Intrahepatic Cholestasis of Pregnancy

目的探讨胎盘ABCB4表达在妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)孕妇妊娠结局预测中的临床价值。方法选取60例ICP孕妇为实验组,同期60例产检正常孕妇为对照组,提取2组产妇分娩后胎盘组织,分析胎盘ABCB4 mRNA和蛋白表达水平,比较2组产妇妊娠结局。结果实验组胎盘组织内ABCB4 mRNA水平(7.58±1.65)低于对照组(26.35±3.54)(P<0.05),ABCB4蛋白阳性表达率(53.33%)低于对照组(95%)(P<0.05)。实验组羊水污染、胎儿窘迫、早产、新生儿窒息等不良妊娠结局发生率高于对照组(P<0.05)。结论ICP孕妇胎盘ABCB4基因、蛋白阳性表达率呈低表达,胎盘ABCB4表达水平可能是预测妊娠结局的参考指标。

Objective To investigate the clinical significance of placental ABCB4 expression in predicting pregnancy outcome in pregnant women with intrahepatic cholestasis of pregnancy(ICP).Methods A total of 60 cases of ICP pregnant women were selected as the experimental group,and 60 cases of normal pregnant women as the control group.The placental tissues were extracted from the two groups after delivery,and the expression levels of ABCB4 mRNA and protein were analyzed.Results The mRNA level of ABCB4 in the experimental group was lower than that in the control group(7.58±1.65 vs.26.35±3.54,P<0.05),and the positive expression rate of ABCB4 protein was lower than that in the control group(53.33%vs.95%,P<0.05).The incidence of adverse pregnancy outcomes such as amniotic fluid contamination,fetal distress,premature birth and neonatal asphyxia in the experimental group was higher than that in the control group(P<0.05).Conclusion The expression rate of ABCB4 gene and protein in placenta of pregnant women with ICP is low.The expression level of ABCB4 in placenta may be a reference index for predicting pregnancy outcome.