猴头菌β-葡聚糖-壳聚糖纳米颗粒制备及其体外生物活性

Preparation and in vitro Biological Activity of Sulfated Hericium erinaceus β-Glucan-Chitosan Nanoparticles

采用自组装的方法通过静电相互作用制备硫酸化猴头菌β-葡聚糖-壳聚糖纳米颗粒(sulfated Hericiumerinaceusβ-glucan-chitosan nanoparticles,DS-CSNPs),以粒径为指标,通过单因素实验和响应面分析,优化DS-CS NPs的制备工艺,并评价最佳工艺条件下纳米颗粒的粒径、形态以及体外生物活性。结果表明,纳米颗粒最佳制备工艺参数:硫酸化猴头菌β-葡聚糖(sulfated H.erinaceusβ-glucan,DS)质量浓度为1mg·mL^(-1),搅拌速度为800 r·min^(-1),壳聚糖(chitosan,CS)初始pH为4,DS-CS NPs的粒径为128.41 nm,且分布较窄。与DS相比,DS-CS NPs体外抗氧化活性显著提高,同时,DS-CS NPs对LPS诱导的巨噬细胞RAW 264.7产生NO以及促炎因子TNF-α分泌有显著抑制作用,并呈浓度依赖性。硫酸化猴头菌β-葡聚糖-壳聚糖纳米颗粒具有体外抗氧化和抗炎活性。

Using the self-assembly method, nanoparticles of sulfated Hericium erinaceus β-glucan-chitosan(DS-CS NPs) were prepared through electrostatic interaction. The preparation process of DS-CS NPs was optimized by single factor experiments and response surface design with particle size as the evaluation index.The nanoparticles prepared under the optimal process were determined for particle size, morphology and in vitro bioactivity. The results showed that the optimal nanoparticle preparation parameters were as follows: 1 mg·mL^(-1)sulfated H. erinaceus β-glucan(DS), 800 r·min^(-1) stirring rate, initial pH of chitosan(CS) solution set at pH 4. The resultant DS-CS NPs were 128. 41 nm in average with a narrow particle distribution. Compared with DS, DS-CS NPs showed higher antioxidant activity in vitro. DS-CS NPs significantly inhibited LPS-induced NO production and TNF-α secretion in macrophage RAW264.7 cells in a concentration-dependent manner. Sulfated H. erinaceus β-glucan-chitosan nanoparticles showed antioxidant and anti-inflammatory activities in vitro.