EZH2抑制剂EPZ-6438对胃癌细胞侵袭和转移的作用研究

The Study on the Effect of EZH2 Inhibitor EPZ-6438 on Invasion and Metastasis of Gastric Cancer Cells

目的探讨EZH2抑制剂EPZ-6438对胃癌细胞侵袭和转移的作用。方法通过Transwell筛选侵袭力高的胃癌细胞HGC-27作为研究对象,根据前期MTT实验选择EPZ-6438的作用浓度为0、5、10、15μmol/L,用Transwell试验和细胞划痕实验检测EPZ-6438对胃癌细胞HGC-27侵袭和转移的影响。Western Blot检测E-cadherin、N-cadherin、Vimentin以及基质金属蛋白酶MMP-2和MMP-9的表达。结果Transwell试验和细胞划痕实验表明,随着EPZ-6438浓度的增加,与对照组相比,胃癌细胞HGC-27的侵袭和转移能力明显下降。Western Blot实验结果表明,与对照组相比,胃癌细胞HGC-27中E-cadherin的表达升高,而N-cadherin和Vimentin的表达降低,MMP-2和MMP-9的表达也呈浓度梯度降低。结论EZH2抑制剂EPZ-6438能有效抑制胃癌细胞HGC-27的侵袭和转移,其可能是通过抑制胃癌细胞HGC-27的上皮间质转化(EMT)来实现的。

Objective To investigate the effect of EZH2 inhibitor EPZ-6438 on the invasion and metastasis of gastric cancer cells.Methods The highly invasive gastric cancer cell line HGC-27 was screened by Transwell as the research object.The concentration of EPZ-6438 was 0,5,10 and 15μmol/L according to the previous MTT experiment.Transwell assay and wound healing assay were used to detect the effect of EPZ-6438 on the invasion and metastasis of gastric cancer cell line HGC-27.Western Blot was used to detect the expression of E-cadherin,N-cadherin,Vimentin and matrix metalloproteinases MMP-2 and MMP-9.Results Transwell assay and cell scratch assay showed that with the increase of EPZ-6438 concentration,the invasion and metastasis ability of gastric cancer cell HGC-27 significantly decreased compared with the control group.Western Blot results showed that compared with the control group,the expression of E-cadherin in gastric cancer cells HGC-27 was increased,while the expression of N-cadherin and Vimentin was decreased,and the expression of MMP-2 and MMP-9 was also decreased in a concentration gradient.ConclusionEZH2 inhibitor EPZ-6438 can effectively inhibit the invasion and metastasis of gastric cancer cells HGC-27,which may be achieved by inhibiting the epithelial-mesenchymal transition(EMT)of gastric cancer cells HGC-27.