分子印迹聚合物吸头整体柱固相萃取结合GC-MS测定化妆水中的局麻药

Determination of Local Anesthetics in Toner Sample Using Molecularly Imprinted Polymer Based Monolith Pipette Tip for Solid-Phase Extraction Couple with GC-MS

利用分子印迹吸头整体柱结合便捷、高效固相萃取装置与萃取方法对化妆水中5种局麻药进行探究,并采用GC-MS实现了准确、灵敏的测定.以布比卡因分子印迹聚合物(MIPs)为选择性吸附剂,超高分子量聚乙烯为粘结剂,在移液吸头中通过共烧结制备了分子印迹吸头整体柱固相萃取(PT-SPE)装置.当pH=9.00、10%乙腈水为上样溶剂、甲醇为洗脱溶剂时,PT-SPE对化妆水中局麻药的萃取率最高,且处理后样品基质干扰显著降低,谱图变“干净”,5种局麻药LOQs为0.06~0.09 mg·L^(-1),该样品前处理方法简化了对化妆水样品的萃取步骤,有效地提高了对化妆水样品测定的灵敏度,实现了对5种局麻药的准确测定.

In this paper,a convenient and efficient pipette tip solid-phase extraction(PT-SPE)device and corresponding method were developed and coupling it with GC-MS enabled accurate and sensitive determination of local anesthetics in toner.PT-SPE device was fabricated by co-sintering of bupivacaine molecularly imprinted polymers(MIPs)as selective adsorbent and ultra-high molecular weight polyethylene as co-sintering agent.The optimal extraction conditions for local anesthetics in toner sample by PT-SPE were pH=9.00,10%acetonitrile water as the loading solvent and methanol as the elution solvent.Under these conditions,a clean chromatogram of the sample was obtained,proving that the treated sample contains less matrix interference and the LOQs of local anesthetics were 0.06~0.09 mg·L^(-1).The sample pretreatment method simplified the extraction steps,effectively improved the sensitivity of the determination of the toner sample,and enabled the accurate determination of five local anesthetics.