人参皂苷Re对淋巴管内皮细胞VEGF-C表达及其功能的影响

Effects of ginsenoside Re on the expression of VEGF-C and function in lymphatic endothelial cells

目的:通过观察人参皂苷Re干预后对淋巴管内皮细胞(LECs)血管内皮生长因子-C(VEGF-C)表达影响,探索人参皂苷Re促进淋巴管生成作用,为人参皂苷Re成为治疗淋巴水肿潜在药物提供依据。方法:使用CCK-8法测定不同浓度人参皂苷Re干预24 h后对LECs活性影响。运用ELISA方法检测细胞上清液中VEGF-C含量。采用RT-qPCR和Western blot方法,检测不同浓度人参皂苷Re干预下LECs VEGF-C mRNA及蛋白表达。通过划痕实验与成管实验,观察人参皂苷Re对LECs功能影响。结果:0~50μmol/L的人参皂苷Re干预24 h后对LECs活性无显著性影响。人参皂苷Re可以刺激VEGF-C mRNA表达,增加其蛋白表达及上清液中含量,同时促进LECs迁移和成管,并呈现出剂量依赖性。结论:人参皂苷Re通过刺激LECs VEGF-C表达,发挥促进淋巴管生成作用,是潜在治疗淋巴水肿药物。

Objective:To study the effects of ginsenoside Re on the expression of VEGF-C in lymphatic endothelial cells(LECs),to investigate the effects of ginsenoside Re on lymphangiogenesis,and to provide evidence for ginsenoside Re to become a potential drug for the treatment of lymphatic system-related diseases.Methods:LECs were treated with different concentrations of ginsenoside Re.After 24 h of intervention,the CCK-8 assay was used to determine the activity of LECs.The levels of VEGF-C in the cell supernatant were detected by ELISA.The expression level of VEGF-C was measured by RT-qPCR and Western Blot,and the function of LECs was showed by wound healing and tube formation assays.Results:The O~50μmol/L ginsenoside Re had no effect on the activity of LECs.Ginsenoside Re can stimulate VEGF-C mRNA,protein expression and supernatant content,and promote the migration and tube formation of LECs in a dose-dependent manner.Conclusion:Ginsenoside Re can promote lymphangiogenesis through stimulating the expression of VEGF-C in LECs.