兔胫骨钻骨孔径对骨髓炎模型的影响

Size of the borehole affects rabbit tibial osteomyelitis models

背景:直到现在骨髓炎仍然是临床骨科医生需要解决的难题,开发理想的骨髓炎模型对于新治疗方法的评价非常重要。目的:稳定有效地制备出适用于不同实验需求的兔胫骨骨髓炎模型。方法:将25只新西兰大白兔随机分成3组,小孔组10只、大孔组10只、假手术组5只。小孔组采用小孔法,用1 mm钻头在兔胫骨中上段钻孔,注入金黄色葡萄球菌(2×10^(6)cfu)悬浮液100μL;大孔组采用大孔法,用5 mm钻头在兔胫骨中上段钻孔,其余操作同小孔组;假手术组切开后缝合,不进行钻孔及细菌注射。术后1个月内进行一般状态、体温、X射线片、CT、组织病理学观察及血清C-反应蛋白、降钙素原质量浓度测定。结果与结论:(1)与假手术组相比,术后小孔组、大孔组兔体温和血清C-反应蛋白质量浓度均呈现不同程度升高;(2)钼靶X射线片及CT检查:小孔组影像学表现为全骨髓腔感染,大孔组表现为局部骨缺损感染;(3)提示在制备兔胫骨骨髓炎模型时,钻孔大小不同可导致不同的胫骨感染后果,使用5 mm钻孔的模型可能适用于局部抗感染材料效能的研究,使用1 mm钻孔的模型可能适用于全身抗生素药物治疗骨髓炎效果的评价。

BACKGROUND:Osteomyelitis remains a challenge for clinical orthopedic surgeons to address until now,and the development of ideal models of osteomyelitis was very important for the evaluation of new treatments.OBJ ECTIVE:To stably and effectively establish a rabbit tibia model of osteomyelitis,suitable for different experimental requirements.METHODS:A total of 25 New Zealand white rabbits were randomly divided into three animal model groups:pore group(n=10),macropore group(n=10)and sham operation group(n=5).In the pore group,a 1 mm drill was used to drill holes in the tibia and the 100μL Staphylococcus aureus(2×10^(6) cfu)was injected.In the macropore group,the same method was used but the drill holes were 5 mm.The sham operation group was cut and sutured without drilling or injecting bacte ria.The general state,body temperature,X-ray,CT,histopathological observation,serum C-reactive protein and procalcitonin mass concentration were measured within 1 month after surgery.RESULTS AND CONCLUSION:Compared with the sham operation group,the body temperature and serum C-reactive protein mass concentration of rabbits in the pore group and the macropore group increased in varying degrees.Molybdenum target X-ray film and CT examination:The imaging manifestations of the pore group were whole marrow cavity infection,while the macropore group was local bone defect infection.It is suggested that in the preparation of rabbit models of tibial osteomyelitis,the size of the borehole may lead to different consequences of tibial infection.The 5 mm borehole model may be suitable for the study of the efficacy of local anti-infection materials,and the 1 mm borehole model may be suitable for the evaluation of the efficacy of systemic antibiotic drug treatment of osteomyelitis.