藤葛复合固体饮料对急性酒精中毒小鼠的解酒护肝作用

Anti-alcoholic and hepatoprotective effects of complex solid beverage from Ampelopsis grossedentata and Puerariae fos extracts in mice with acute alcoholism

目的研究藤葛复合固体饮料对急性酒精中毒小鼠的解酒作用和护肝功能。方法各实验组灌胃相应受试物后建立急性小鼠醉酒模型,记录小鼠防醉实验行为学变化,检测血清乙醇含量、谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)和甘油三酯(triglyceride,TG)水平,测定体质量、肝脏指数、肝脏乙醇代谢酶活力、抗氧化指标水平、肿瘤坏死因子-α(tumornecrosisfactor-α,TNF-α)和抑炎因子白细胞介素-6(interleukin-6,IL-6)水平,镜检观察肝脏病理学变化。结果与模型组相比,藤葛复合解酒固体饮料低、高剂量组均可显著延长小鼠酒精耐受时间(P<0.05,P<0.01),缩短睡眠时间(P<0.01)和醒酒时间(P<0.05,P<0.01);低、高剂量组均能极显著降低小鼠血液中乙醇浓度、ALT、AST和TG水平(P<0.01);高剂量组显著提高小鼠体质量(P<0.05)和减轻肝脏指数(P<0.01);高剂量组能极显著提高乙醇脱氢酶(alcohol dehydrogenase,ADH)、乙醛脱氢酶(acetaldehyde dehydrogenase,ALDH)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活力、谷胱甘肽过氧化物酶活力(glutathione peroxidase,GSH-Px)、还原型谷胱甘肽(glutathione,GSH)含量(P<0.01)和降低肝脏丙二醛(malondialdehyde,MDA)含量(P<0.01);低、高剂量组均能明显改善肝组织病理情况,抑制肝脏中TNF-α和IL-6水平(P<0.01)。结论藤葛复合固体饮料对急性酒精中毒小鼠具有较好的解酒护肝作用,可能与其提高小鼠乙醇代谢酶活力加速乙醇代谢分解、机体抗氧化能力增强、炎症信号的传导抑制有关。

Objective To study the anti-alcoholic effect and hepatoprotective function of the compound solid beverage of Ampelopsis grossedentata and Puerariae fos on acute alcoholism mice.Methods After gavaged of the corresponding test substances in each experimental group,an acute intoxication model was established in mice,recorded the behavioral changes in the anti-intoxication test of mice,measured serum ethanol concentration,serum alanine aminotransferase(ALT),aspartate aminotransferase(AST)and triglyceride(TG)levels,and determined the body quality and liver index,liver alcohol metabolism enzyme activity,antioxidant index levels,tumor necrosis factor-α(TNF-α)and anti-inflammatory factor interleukin-6(IL-6)levels,and the pathological changes of the liver were observed by microscopy.Results Compared with the model group,both the low and high dose groups of the compound solid beverage of Ampelopsis grossedentata and Puerariae fos could significantly prolong the alcohol tolerance time(P<0.05,P<0.01),shorten the sleep time(P<0.01)and sober up time(P<0.05,P<0.01);both low-dose and high-dose groups could significantly reduce the blood ethanol concentration,ALT,AST and TG levels of mice(P<0.01);the high-dose group significantly increased the body weight of mice(P<0.05)and relieved the liver index of mice(P<0.01);the high-dose group could significantly increase alcohol dehydrogenase(ADH),acetaldehyde dehydrogenase(ALDH),superoxide dismutase(SOD),catalase(CAT)activity,glutathione peroxidase(GSH-Px)and glutathione(GSH)content(P<0.01),reduced liver malondialdehyde(MDA)content(P<0.01).The low and high-dose group could significantly improve the pathological conditions of liver tissue,inhibite the levels of TNF-αand IL-6 in the liver(P<0.01).Conclusion The compound solid beverage of Ampelopsis grossedentata and Puerariae fos has better anti-alcoholic and hepatoprotective effects on acute alcoholism mice,which may be related to accelerate ethanol metabolism,enhance the mice’s antioxidant capacity,and inhibite the transduction of inflammatory signals.