用于葵花籽过氧化值和酸价检测的油脂提取工艺优化

Optimization of oil extraction process for determining peroxide value and acid value of sunflower seeds

目的优化葵花籽过氧化值和酸价检测的油脂制备工艺。方法借助超声优化油脂制备工艺,以油脂得率为评价指标,在单因素实验的基础上,采用Plackett-Burman设计和Box-Behnken Design响应面法对影响因素进行优化,然后用优化后的工艺与国家标准方法对6种葵花籽进行油脂提取并比较分析其油脂得率、过氧化值和酸价,评价优化方法可行性。结果优化的超声最佳提取工艺为粉碎目数70目、料液比1:4(g:mL)、浸提时间2h、超声功率312W、超声时间37min,经验证此优化工艺提取的油脂得率比国家标准方法提高19%~25%,油脂制备时间由12 h以上缩短至2.5 h,缩短了80%的制备时间,提取效率更高,且测得的过氧化值结果更低。结论优化的超声工艺提取油脂更多且耗时短,不仅满足了检测用油量的需求,还降低了长时间油脂提取的氧化风险,检测结果更准确,更能真实反映葵花籽的品质,经验证此方法准确可行,可用于葵花籽过氧化值和酸价检测的油脂提取制备。

Objective To optimize the oil preparation process for determining peroxide value and acid value of sunflower seeds.Methods The preparation process of that oil was optimized by mean of ultrasound,the yield of the oil was taken as an evaluation index,on the basis of single factor experiment,Plackett-Burman design and Box-Behnken Design response surface method were used to optimize the influencing factors.Then,6 kinds of sunflower seeds were extracted by the optimized technology and national standard method,their oil yield,peroxide value and acid value were compared and analyzed,and the feasibility of the optimization method was evaluated.Results The optimized ultrasonic extraction method was as follows:Grinding mesh 70,solid-liquid ratio 1:4(g:mL),extraction time 2 h,ultrasonic power 312 W and ultrasonic time 37 min.It had been proved that the oil yield of this optimized process was increased by 19%-25%compared with the national standard method,and the oil preparation time was shortened from more than 12 h to 2.5 h,which shortens the preparation time by 80%.The extraction efficiency was higher,and the measured peroxide value was lower.Conclusion The optimized ultrasonic technology can extract more oil and consume less time,which not only meets the demand of oil consumption detection,but also reduces the oxidation risk of long-term oil extraction.The detection result is more accurate,and it can truly reflect the quality of sunflower seeds.It has been verified that this method is accurate and feasible,and can be used for oil extraction for determining peroxide value and acid value of sunflower seeds.