基于网络药理学的右归胶囊治疗弱精症的作用机制研究

Study on the mechanism of action of Yougui Capsule in the treatment of asthenospermia based on network pharmacology

目的 使用网络药理学的方法,探寻右归胶囊治疗弱精症的分子机制。方法 通过中国知网查找近5年(2016年3月29日至2021年3月29日)的相关文献获得右归胶囊内各中药的相关成分,利用Swiss Target Prediction平台筛选出右归胶囊内各中药的主要有效化合物及其靶点;运用Cytoscape 3.8绘制药物-成分-靶点网络;通过Genecards、OMIM数据库检索弱精症相关靶点;通过韦恩图得到右归胶囊、弱精症的共同靶点;使用String平台构建化合物-疾病共同靶点的蛋白互作网络;使用Metascape数据库进行基因本体分析和京都基因与基因组百科全书(KEGG)通路分析。结果 共获得右归胶囊内各中药的主要成分45个,418个弱精症相关靶点,右归胶囊调节弱精症的作用靶点11个,GO富集分析共得主要条目中涉及生物过程14条。KEGG通路分析共得到核心信号通路1条,即细胞周期通路。结论 右归胶囊可能通过多种有效成分作用于共同核心靶点,从而提高精子活力而治疗弱精症。

Objective To explore the molecular mechanism of Yougui Capsule in the treatment of asthenospermia using a network pharmacology approach. Methods Through searching relevant literatures in recent 5 years(March 29, 2016-March 29, 2021) on CNKI, the relevant components of TCM in Yougui Capsule were obtained, and the main effective compounds and their targets of TCM in Yougui Capsule were screened using the Swiss Target Prediction Platform. Cytoscape 3.8 was used to construct drug-component-target network. Targets related to asthenospermia were retrieved through Genecards and OMIM databases. The common target of Yougui Capsule and asthenospermia was obtained by Wayne diagram. The String platform was used to construct the protein interaction network of compound and disease common target. Metascape database was used for gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis. Results A total of 45 main components of traditional Chinese medicine and 418 targets related to asthenospermia were obtained, and 11 targets related to the regulation of asthenospermia were obtained in Yougui capsule. Among the main items, 14 biological processes were identified by GO enrichment analysis. KEGG pathway analysis revealed a core signaling pathway, namely the cell cycle pathway. Conclusions Yougui capsule may treat asthenospermia by acting on the common core target with a variety of active ingredients to improve sperm motility.