深县猪催乳素受体(PRLR)基因SNPs位点多态性及其与繁殖性能的相关分析

SNPs polymorphism of PRLR gene and its correlation with reproductive performance in Shenxian pigs

试验旨在研究催乳素受体(PRLR)基因SNPs位点的多态性对深县猪繁殖性能的影响。试验通过PCR-RFLP技术结合混合池一代测序技术检测PRLR基因的SNPs位点,并与71头深县猪的繁殖性能进行关联分析。结果显示,PRLR基因在深县猪群体中有两个SNPs位点:A133T和T225C,均位于第8外显子。A133T位点上存在3种基因型:AA基因型、AT基因型和TT基因型,其中AA基因型为优势基因型,A为优势等位基因,AA基因型猪的总产仔数、初生窝重、产活仔数均显著高于TT基因型个体(P<0.05)。T225C位点上存在3种基因型:TT基因型、TC基因型和CC基因型,其中C为优势等位基因,CC为优势基因型,TC基因型和CC基因型猪的总产仔数、初生窝重均显著高于TT基因型个体(P<0.05)。合并基因型中,ATTC和ATCC基因型的繁殖性能总体较高。两个位点均处于哈迪-温伯格平衡状态,多态信息含量分别为0.329、0.373,为中度多态;杂合度分别为0.416、0.495;纯合度分别为0.584和0.505。研究表明,可以通过选育A133T位点的AA基因型和T225C位点的TC基因型个体,合并基因型中的ATTC和ATCC基因型个体提高深县猪的繁殖性能。

The study was to study the effect of prolactin receptor(PRLR)gene SNPs polymorphism on reproductive performance in Shenxian pigs.The SNPs locus of PRLR gene was detected by PCR-RFLP technique combined with mixed pool generation sequencing technology,and association with the reproductive performance of 71 multiparous sows of shenxian pigs was analyzed.The results showed that there were two SNPs loci of PRLR gene in Shenxian pig population:A133T and T225C,both located in exon 8.There were three genotypes at A133T:AA genotype,AT genotype and TT genotype,in which AA genotype was the dominant genotype and A is the dominant allele.The total litter size,litter weight and live litter size of AA genotypes were significantly higher than those of TT genotypes(P<0.05).There were three genotypes at T225C:TT,TC and CC,in which C was the dominant allele and CC was the dominant genotype.The total litter size and litter weight of TC and CC genotypes were significantly higher than those of TT genotypes(P<0.05).Among the combined genotypes,ATTC and ATCC genotypes had higher reproductive performance as a whole.The two loci were in Hardy-Weinberg equilibrium.The polymorphism information content was 0.329 and 0.373,respectively.The heterozygosity was 0.416 and 0.495,and the homozygosity was 0.584 and 0.505,respectively.The study indicates that the reproductive performance of Shenxian pigs can be improved by selecting individuals with AA genotypes at A133T site and TC genotypes at T225C loci,and combining individuals with ATTC and ATCC genotypes.