电荷转移反应共振光散射光谱法测定酒石酸泰乐菌素含量方法的建立

Establishment of a method for the determination of tylosin tartrate by resonance light scattering spectrometry for charge transfer reaction

研究旨在建立一种快速检测酒石酸泰乐菌素含量的光谱法。使用共振光散射光谱法测定pH值为6.4条件下,酒石酸泰乐菌素与阳离子染料中性红发生反应前后引起的共振光散射光谱的强度差。结果显示:在扫描速度为2000 nm/min、激发光和发射光带宽均为5.0 nm时,酒石酸泰乐菌素与阳离子染料中性红在Clark-Lubs缓冲溶液中发生电荷转移反应,形成电荷转移配合物使该体系的共振光散射强度减弱。中性红的共振光散射光谱峰位于324 nm时,该体系的共振散射光谱的强度差值达到最大,在0.05~0.60 mg/L内呈线性关系,相关系数R为0.9996,检测限(3 d/k)为0.0115 mg/L。研究表明,试验所用方法简便、灵敏度高,可应用于测定酒石酸泰乐菌素含量。

The study was to establish a fast spectrometric method for determination of tylosin tartrate.The intensity difference of resonance light scattering spectra before and after the reaction of tylosin tartrate with cationic dye neutral red was measured by resonance light scattering spectroscopy under the condition of pH value 6.4.The results showed that when the scanning speed was 2000 nm/min and the excitation light and emission light bandwidth were both 5.0 nm,the charge transfer reaction occurred between tylosin tartrate and the cationic dye neutral red in Clark-Lubs buffer solution,and the charge transfer complex was formed,which weakened the resonance light scattering intensity of the system.When the resonance light scattering spectrum peak of neutral red is located at 324 nm,the intensity difference of the resonance scattering spectrum of the system reaches the maximum,with a linear relationship between 0.05 and 0.60 mg/L,and the correlation coefficient R is 0.9996.The detection limit(3 d/k)of the test method was 0.0115 mg/L.The study indicates that the method is simple and sensitive,and can be used to determine the content of tylosin tartrate.