不同培养条件及其组合对杉木无性系T-c22组培苗不定根诱导的影响

Effects of different culture conditions and their combinations on adventitious rootinduction of tissue culture seedlings of Chinese fir clone T-c22

【目的】不定根诱导是杉木优良无性系繁殖的重要手段,杉木组织培养中不定根诱导受到多种因素影响,但起主导作用的关键因素尚不清楚。本研究通过比较不同培养条件对杉木不定根诱导的影响,以期研究出最适宜杉木生根的培养因素,为后续试验和研究奠定基础。【方法】以杉木T-c22无性系为试验材料,通过改变营养物质条件、植物生长调节剂种类及浓度、去除针叶情况、有无活性炭、光照条件以及不同光质等不同培养因素,统计杉木生根的各种指标,研究不同培养因素以及不同因素与光照之间的交互作用对杉木生根的影响。【结果】(1)光照比黑暗显著提高杉木组培苗的株高、生根率、平均根数以及平均根长,显著抑制杉木的根鲜质量。(2)白光和红光均能提高杉木的生根率,生根率达100%;白光能够显著提高杉木的生根数量;红光和蓝光均会显著降低杉木的根长,但能够显著提高杉木无菌苗的株高。(3)添加IBA、NAA等单一植物生长调节剂显著提高杉木的株高、平均生根数量、平均根长、根鲜质量以及根干质量;光照和单一生长调节剂的交互作用显著提高平均根数以及平均根长。(4)培养基中含有适量营养成分会显著提高杉木的生根率、生根数量、平均根长、平均根鲜质量以及平均根干质量;光照和有无营养的交互作用显著提高杉木的株高和平均根长。(5)去针叶显著提高杉木无菌苗株高、根的平均长度及平均干质量;光照和有无去针叶的交互作用对杉木不定根的诱导没有显著的影响。(6)活性炭显著降低杉木无菌苗的生根率、生根数量、根鲜质量以及根干质量;活性炭和光照的交互作用显著降低杉木的生根率以及平均根长。【结论】本研究中影响杉木T-c22无性系生根的因素重要性排序为:营养物质>植物生长调节剂的种类和浓度>不同光质>光照时间以及光照与其他因素的交互作用>有无去针叶和活性炭。最适宜杉木T-c22无性系生根的培养基配方为1/2MS+NAA 0.5 mg/L+IBA 0.5 mg/L;最适宜杉木生根的培养条件为添加适宜的营养物质、适宜浓度的植物生长调节剂(NAA 0.5 mg/L)、去除部分针叶以及红光(16 h/d)处理。

[Objective]Adventitious root induction is an important method for the propagation of superior clones of Cunninghamia lanceolata.Adventitious root induction in C.lanceolata tissue culture is affected by many factors.However,the key factors that play a leading role are still unclear.By comparing the influences of different culture conditions on the induction of C.lanceolata adventitious roots,this study aimed to find out the most suitable culture factors for C.lanceolata rooting,laying a foundation for subsequent experiments and studies.[Method]Using C.lanceolata clone T-c22 as the experimental material,by changing the nutrient conditions,the types and concentrations of plant growth regulator(hormone),removing needle leaves from shoots,adding activated carbon to the medium,light conditions and different light quality and other different culture factors,and various indexes of C.lanceolata rooting were investigated and statistically analyzed,in order to study the influence of different culture factors and the interaction between different factors and light on the rooting of C.lanceolata.[Result](1)The results showed that compared with darkness,light significantly increased the plant height,rooting rate,average root number and average root length of C.lanceolata shoots,and significantly inhibited the fresh root mass of C.lanceolata.(2)Both white light and red light can increase the rooting rate of C.lanceolata,reaching 100%.White light can significantly increase the number of roots of C.lanceolata.Both red light and blue light can significantly reduce the root length of C.lanceolata.And they can significantly increase the plant height of plantlets of C.lanceolata.(3)Adding IBA and NAA single hormone significantly increased the plant height,average number of roots,average root length,root fresh mass and root dry mass of C.lanceolata.The interaction of light and a single hormone significantly increased the average root number and average root length.(4)The medium containing appropriate nutrients will significantly increase the rooting rate,number of roots,average root length,average fresh root mass and average root dry mass of C.lanceolata.The interaction of light and the nutrition significantly increased the plant height and average root length of C.lanceolata.(5)Removal of needles significantly increased the height of plantlets,the average root length and the average dry mass of roots.The interaction of light and removing needle leaves from shoots had no significant effect on the induction of adventitious roots of C.lanceolata.(6)Activated carbon significantly reduced the rooting rate,number of roots,fresh root mass and root dry mass of C.lanceolata plantlets.The interaction of activated carbon and light significantly reduced the rooting rate and average root length of C.lanceolata.[Conclusion]In this study,the importance order of factors affecting rooting of T-c22 clones of C.lanceolata was as follows:nutrients>species and concentration of plant growth regulators>different light quality>light duration and the interaction between light and other factors>whether or not to remove needles and activated carbon.The optimal medium for rooting of T-c22 clone of C.lanceolata was 1/2MS+NAA 0.5 mg/L+IBA 0.5 mg/L.The optimal rooting conditions for C.lanceolata were the addition of appropriate nutrients,the appropriate concentration of plant growth regulator(NAA 0.5 mg/L),the removal of some needles and the treatment of red light(16 h/d).