不同剂量的枸杞沙棘配伍对小鼠免疫功能的影响

Effects of different doses of Lycium chinensis and seabuckthorn on immune function of mice

为探索枸杞和沙棘不同配伍比例对小鼠免疫功能的影响,经口给予小鼠0.35、0.70、1.05 g/kg·BW剂量的枸杞沙棘胶囊粉30 d,分别测定免疫器官脏器/体重比值。采用刀豆蛋白(concanavalin A,ConA)诱导的小鼠脾淋巴细胞转化实验、2,4-二硝基氟苯(2,4-dinitrofluorobenzene,DNFB)诱导的小鼠迟发型变态反应、血清溶血素的测定、小鼠碳廓清实验、抗体生成细胞检测、自然杀伤细胞(natural killer cell,NK细胞)活性测定、小鼠腹腔巨噬细胞吞噬鸡红细胞实验等方法测定小鼠各项指标。结果表明,与阴性对照组比较,高剂量组能够显著增强ConA诱导的小鼠脾淋巴细胞转化能力及DNFB诱导的迟发型变态反应(delayed type hypersensitivity,DTH)、吞噬率、吞噬指数、小鼠碳廓清吞噬指数、NK细胞活性及抗体生成细胞数量,且差异显著(P<0.05);中剂量组可显著提高小鼠抗体积数,差异显著(P<0.05),说明该产品对细胞免疫功能和体液免疫功能测定结果均为阳性,具有增强免疫力的功效。该研究证明枸杞和沙棘配伍可用于提高免疫力产品的开发。

To explore the effects of different doses of Lycium barbarum and seabuckthorn on the immune function of mice,mice were orally given 0.35,0.70,and 1.05 g/kg·BW of Lycium barbarum and seabuckthorn capsule powder for 30 d,and the organs/body weight ratio was measured respectively.The indexes of mice were determined by ConA-induced spleen lymphocyte transformation test,delayed-type hypersensitivity(DTH)induced by DNFB,serum hemolysin test,carbon clearance ability test,antibody-producing cells test,NK cell activity test,and peritoneal macrophage phagocytosis of chicken red blood cells test.Results showed that compared with the negative control group,the high-dose group(1.05 g/kg·BW)could significantly enhance the ConA-induced splenic lymphocyte transformation ability,DTH reaction induced by DNFB,phagocytosis rate,phagocytosis index,carbon clearance phagocytosis index,NK cell activity and the number of antibody-producing cells,and the differences were significant(P<0.05).The middle-dose group(0.70 g/kg·BW)could significantly increase the number of antibodies in mice,and the differences were significant(P<0.05),which indicated that the product was positive for both cellular and humoral immune function,and had the effect of enhancing immunity.This study finally proved that the compatibility of Lycium barbarum and seabuckthorn could be used to develop immunity-enhancing products.