埃希氏菌的遗传演化关系分析

Analysis of genetic evolution relationship of Escherichia

为了探究不同国家和地区埃希氏菌(Escherichia)的遗传演化规律,明确不同国家和地区埃希氏菌的亲缘关系,试验采用生物信息学方法,利用细菌16S r RNA测序技术,对实验室分离和NCBI中搜索得到的16S rRNA基因进行整理、修剪后构建分子进化树进行分析比较。结果表明:埃希氏菌属各种间存在着一定的亲缘关系,存在着程度不同的变异。埃希氏菌属不同种间变异差别较大,大肠埃希氏菌和弗格森埃希氏菌关系密切,之间最易变异;艾伯特埃希氏菌在大范围内相对保守,且在亚洲与大洋洲、南极洲三大洲间存在着交叉进化;旱獭埃希氏菌在中国、印度和韩国之间呈现两枝交叉进化,旱獭埃希氏菌与伤口埃希氏菌关系密切。研究结果为阐明埃希氏菌的分子流行病学提供参考依据。

In order to explore the genetic evolution of Escherichia in different countries and regions, and to clarify the genetic relationship of E. in different countries and regions, bioinformatics methods were used to analyze and compare the 16S rRNA genes obtained from laboratory isolation and NCBI, and construct molecular evolutionary tree after pruning. The results showed that there was a certain genetic relationship between the strains of E. coli, which may be the relationship between ancestors and offspring, there were varying degrees of variation, and the ability of variation among different strains had different levels. The transformation ability of E. coli and E. fergusonii was the strongest, and the two strains evolved from the same original strain in a very early period. E. albertii strains coexisted in the same region with highly conservative strains, and cross-evolution existed among Asia, Oceania and Antarctica. E. marmotae and E. vulneris strains originated in the Asian continent, and E. marmotae strains from China were clustered into three groups. E. Marmota had two branches of cross evolution in China, India and South Korea. E. marmota was closely related to E. vulneris. The results provide reference for elucidating the molecular epidemiology of Escherichia.