水稻‘9311'突变体库构建及磷响应突变体筛选

Construction of Rice '9311' Mutant Library and Screening of Mutants Res- ponse to Phosphorus

水稻是全球最重要的粮食作物之一,水稻产量的高低关系着全球社会的稳定与发展。磷元素是许多重要生物大分子的关键组分,且参与植物体内许多的生理生化反应,因此植物的生长和发育都离不开磷元素。目前,水稻中有关磷素吸收利用的基因已被挖掘且相关的分子机理研究也在不断深入,同时也取得了一些突破性的进展。但是,水稻磷素吸收利用的调控通路研究相对较复杂,尚需进一步挖掘和解析更多磷利用相关基因的功能。本研究通过设置高磷和低磷水平共筛选了1 525份水稻‘9311’突变体材料,获得了9份响应不同磷水平的差异候选材料,进一步对候选材料分析发现仅有190286突变体能够很大程度上响应磷水平变化且恢复其表型。通过分析190286突变体在高磷与低磷及恢复生长条件下相关形态生理指标,发现在低磷下其根长、地上部分及鲜重方面与‘9311’存在显著差异且为磷高效材料。该研究结果为后期水稻磷吸收利用相关基因的挖掘和功能解析奠定了基础,也为后续水稻磷高效育种提供了材料支撑。

Rice is one of the most important food crops in the world.The yield of rice is related to the stability and development of the global.Phosphorus is the key component of many important biological macromolecules,and participates in many physiological and biochemical reactions in plants,so the growth and development of plants are inseparable from phosphorus.At present,the genes related to phosphorus uptake and utilization in rice has been excavated,and the related molecular mechanism has been further studied,some breakthrough progress has been made.However,the study of P uptake and utilization in rice is relatively complex,and more functions of P use-re-lated genes need to be further explored and analyzed.In this study,a total of 1525 rice'9311'mutant lines were screened by setting both high and low P levels and 9 candidate lines were obtained with different responses to dif-ferent P levels.Further analysis of candidate lines showed that only 190286 mutant could largely respond to differ-ent P levels and restore its phenotype.By analyzing the relative morphological and physiological indexes of‘190286' mutant under high P, low P and recovery conditions, the results showed that there were significant differencesin root length, stem length and fresh weight between 190286 and '9311' under the condition of low phosphorus,which laid a foundation for the mining and functional analysis of genes related to phosphorus uptake and utilizationin rice. It also provides materials for high efficient phosphorus breeding in rice.