聚乙二醇干扰素α-2b对慢性乙型肝炎患者血清标志物HBsAg、HBV-pgRNA、HBV-RNA的影响

Effects of peginterferonα-2b on serum markers HBsAg,HBV-pgRNA and HBV-RNA in patients with chronic hepatitis B

目的观察聚乙二醇干扰素α-2b(Peg-IFNα-2b)对慢性乙型肝炎(CHB)患者血清乙型肝炎病毒表面抗原(HBsAg)表达水平的影响,并探究其与新型血清标志物乙型肝炎病毒前基因组(HBV-pgRNA)、HBV-RNA的关系。方法收集该院2018年2月至2021年12月初次确诊的36例接受Peg-IFNα-2b治疗6个月的CHB患者血清;使用化学发光法HBsAg测定试剂盒检测血清HBsAg水平;聚合酶链式反应(PCR)荧光探针法检测血清HBV-DNA、HBV-pgRNA、HBV-RNA水平;使用Pearson相关性分析HBV-pgRNA、HBV-RNA与HBsAg之间的相关性。结果与治疗前相比,治疗后CHB患者血清HBsAg、HBV-DNA发生明显降低,HBV-pgRNA、HBV-RNA也明显降低,差异有统计学意义(P<0.05);治疗前血清HBV-pgRNA、HBV-RNA均与HBsAg呈显著的正相关(r=0.751、0.702,均P<0.05);治疗后血清HBV-pgRNA、HBV-RNA与HBsAg也呈正相关(r=0.420、0.625,均P<0.05)。与治疗前相比,治疗后HBV-pgRNA、HBV-RNA与HBsAg的相关系数均明显的降低。结论Peg-IFNα-2b可抑制CHB患者血清HBV-HBsAg,且HBV-pgRNA、HBV-RNA与HBV-HBsAg表达水平呈正相关。

Objective To observe the effect of pegylated interferonα-2b(Peg-IFNα-2b)on the expression of serum HBsAg in patients with chronic hepatitis B(CHB),and explore its relationship with new serum markers hepatitis B virus(HBV-pgRNA),HBV-RNA.Methods Thirty six patients initially diagnosed in Anning first people′s hospital from February 2018 to December 2021 received Peg-IFNα-2b serum of CHB patients treated for 6 months.The content of serum HBsAg was detected by chemiluminescence hepatitis B virus surface antigen(HBsAg)assay kit.The contents of HBV-DNA,HBV-pgRNA and HBV-RNA in serum were detected by PCR fluorescence probe.Pearson correlation was used to analyze the correlation between HBV-pgRNA,HBV-RNA and HBsAg.Results Compared with before treatment,the serum HBsAg,HBV-DNA,HBV-pgRNA,HBV-RNA were all significantly decreased in CHB patients after treatment,the difference was statistically significant(P<0.05).Before treatment,serum HBV-pgRNA,HBV-RNA were all significantly positively correlated with HBsAg(r=0.751,0.702,P<0.05).After treatment,serum HBV-pgRNA,HBV-RNA were also positively correlated with HBsAg(r=0.420,0.625,P<0.05).Compared with before treatment,the correlation coefficients of HBV-pgRNA,HBV-RNA and HBsAg significantly decreased after treatment.Conclusion Peg-IFNα-2b could inhibit serum HBV-HBsAg in patients with CHB,and the expression of HBV-pgRNA,HBV-RNA were positively correlated with HBV-HBsAg.