核酸依赖性扩增技术研究现状及展望

Research progress on nucleic acid sequence-based amplification

自19世纪90年代以来,等温核酸扩增技术及其衍生技术迅速发展。等温核酸扩增技术的流程简易、成本和应用要求更低,应用前景广泛。其中,核酸依赖性扩增技术(NASBA)已广泛用于病原体检测、单核苷酸突变检测和环境监测等领域。本文主要对近5年来NASBA技术的研究进展及其在分子诊断中的应用进行综述,探讨NASBA技术的前景与瓶颈。

Since the 1890s,isothermal nucleic acid amplification technology and its derivatives have developed rapidly.The process of isothermal nucleic acid amplification technology is simple,the cost and application requirements are lower,and the application prospect is broad.Among them,nucleic acid sequence-based amplification(NASBA)has been widely used in pathogen detection,single nucleotide mutation detection and environmental monitoring.This paper mainly reviews the research status of NASBA technology and its application in molecular diagnosis in the past five years,and discusses the prospects and bottlenecks of NASBA technology.