苍艾挥发油对左心室重构大鼠的改善作用研究

Ameliorative Effects of Cang-ai Volatile Oil on Left Ventricular Remodeling in Rats

目的采用7T心脏磁共振组织追踪成像(CMR-TT)评估苍艾挥发油(CAVO)对异丙肾上腺素(ISO)所诱导的左心室重构(LVR)模型大鼠的改善作用,并初步探讨其对LVR内皮功能障碍的影响。方法将35只SD雄性大鼠随机分成两组,即正常对照组(8只)和实验组(27只),通过ISO 10 mg·kg-1·d-1连续10 d皮下多点注射建立LVR大鼠模型。造模结束后将实验组存活大鼠(24只)再次随机分为空白实验组、苍艾挥发油组、麝香保心丸组(每组8只),各组连续给予相应药物或等量生理盐水灌胃28 d,分别在造模结束后和灌胃结束后行7T CMR电影序列扫描,并应用后处理软件CVI42进行图像分析,对比各组大鼠用药前后左心室结构和功能参数的变化。磁共振扫描结束后处死大鼠,摘取心脏行病理学检查,ELISA检测血清生化指标。结果苍艾挥发油可明显提高LVR大鼠的左心室射血分数和整体心肌应变参数,降低左心室容积、质量和血清中内皮功能指标水平,同时病理染色显示心肌细胞肥大、坏死以及间质纤维化也得到明显改善。结论CAVO通过对心肌血管内皮功能的调控,可明显改善LVR大鼠心功能,延缓心室重构进程,对大鼠心脏结构和功能具有一定的保护作用。

Objective To evaluate with 7T cardiac magnetic resonance tissue tracking imaging(CMR-TT)the ameliorative effect of Cang-ai volatile oil(CAVO)on left ventricular remodeling(LVR)in rats induced by isoproterenol(ISO),and to make preliminary investigation into CAVO’s effects on endothelial dysfunction in LVR.Methods A total of 35 healthy male Sprague-Dawley(SD)rats were randomly assigned to two groups,the experimental group(n=27)and the normal control group(n=8).The rat model of LVR was established by subcutaneous injection of ISO solution at 10mg·kg-1·d-1at multiple sites for 10 consecutive days.After modeling was completed,the surviving rats(n=24)in the experimental group were then randomly assigned to the blank experimental group,CAVO group,and Shexiang Baoxin pill(SXBXP)group(n=8 in each group).Rats in each group were given via gavage the corresponding intervention medicine or an equivalent amount of normal saline solution for 28 consecutive days.At the end of modeling and intragastric intervention,7T CMR cine sequence scanning was conducted to collect data.Then,post-processing software CVI42 was used to analyze the images and to compare and contrast the changes in the parameters of left ventricular cardiac function and myocardial strain in each group before and after the administration of the medication.The rats were sacrificed after MRI scanning,and their hearts were harvested for pathological examination.The levels of serum biochemical indicators were measured by enzyme-linked immunosorbent assay(ELISA).Results CAVO significantly increased LV ejection fraction and overall myocardial strain parameters in LVR rats,while it decreased LV volume,mass,and serum levels of endothelial function indicators in LVR rats.In addition,pathological staining showed marked improvements in the hypertrophy,necrosis and interstitial fibrosis of cardiomyocytes.Conclusion Through the regulation of myocardial vascular endothelial function,CAVO can significantly improve cardiac functions in LVR rats,delay the process of ventricular remodeling,and have a certain amount of protective effect on cardiac structure and function in rats.