水涝胁迫对草石蚕生理特性的影响及抗涝性评价

Effects of waterlogging stress on physiological characteristics and evaluation of waterlogging resistance of Stachys sieboldii Miq.

以3个草石蚕(Stachys sieboldii Miq.)品种为试材,研究水涝胁迫对草石蚕生理特性的影响,并通过相关性和主成分分析,对草石蚕的抗涝性进行评价,以期为草石蚕抗涝性鉴定提供参考。结果显示,随水涝胁迫时间延长,3种草石蚕叶绿素、可溶性糖、可溶性蛋白含量及超氧化物歧化酶(POD)和过氧化氢酶(CAT)活性呈先升高后降低的变化;“鲁引1号”和“赣引1号”的脯氨酸(Pro)含量先升高后降低,“贵栽1号”Pro含量则一直升高;“鲁引1号”和“贵栽1号”的SOD活性先升高后降低,“赣引1号”则一直升高;随胁迫时间的延长,3种草石蚕的MDA含量均一直增加。相关性分析发现草石蚕的叶绿素含量、可溶性糖含量、Pro含量、SOD活性、POD活性、CAT活性和MDA含量间具有密切的相关性,但相关程度不一致。基于主成分分析的抗涝性综合评价,3种草石蚕的抗涝性为赣引1号>鲁引1号>贵栽1号。

Three Stachys sieboldii Miq.varieties were used to study the effects of waterlogging stress on the physiological characteristics of S.sieboldii Miq..Correlation analysis and principal component analysis were used to evaluate the waterlogging resistance of S.sieboldii Miq.to provide a reference for the identification of waterlogging resistance of S.sieboldii Miq..The results showed that the content of chlorophyll,soluble sugar,soluble protein,and the activity of POD and CAT of the three S.sieboldii Miq.varieties increased first and then decreased with the prolongation of waterlogging stress.The content of Pro in Luyin No.1 and Ganyin No.1 first increased and then decreased,while the content of Pro in Guizai No.1 kept increasing.The activity of SOD in Guizai NO.1 and Luyin No.1 increased first and then decreased,while the activity of SOD in Ganyin No.1 kept increasing under the waterlogging stress.The accumulation of MDA in the three S.sieboldii Miq.varieties increased all the time with the extension of stress time.The results of correlation analyses showed that the content of chlorophyll,soluble sugar,soluble protein,Pro,and MDA,the activity of SOD,POD,and CAT of S.sieboldii Miq.was closely related,but the degree of correlation was inconsistent.The results of comprehensively evaluating waterlogging resistance based on the principal component analysis showed that the waterlogging resistance of the three S.sieboldii Miq.varieties was in the decreasing order of Ganyin No.1>Luyin No.1>Guizai No.1.