半夏厚朴汤治疗咳嗽变异性哮喘的网络药理学研究

Network Pharmacology Research of Banxia Houpu Tang in the Treatment of Cough Variant Asthma

目的:利用网络药理学方法探讨半夏厚朴汤治疗咳嗽变异性哮喘(cough variant asthma,CVA)的作用机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选出半夏厚朴汤中各味中药的活性成分及药物靶点;通过GeneCards数据库获取CVA相关基因,将药物靶点与疾病靶点取交集得到半夏厚朴汤治疗CVA的潜在作用靶点;利用Cytoscape软件建立药物-活性成分-CVA靶点网络图;利用STRING数据库和Cytoscape软件构建蛋白-蛋白相互作用网络(protein-protein interactions,PPI);利用DAVID数据库对半夏厚朴汤治疗CVA的作用靶点进行基因本体论功能富集分析(gene ontology,GO)和KEGG富集分析(kyoto encyclopedia of genes and genomes,KEGG)。结果:共筛选得到44个活性成分,97个半夏厚朴汤治疗CVA的潜在作用靶点,富集分析得到GO功能条目141个,KEGG通路97条。结论:半夏厚朴汤可通过多成分、多靶点治疗CVA。

Objective:To discuss the mechanism of Banxia Houpu Tang in the treatment of CVA by utilizing network pharmacology.Methods:The active ingredients and herbal targets of the herbs in Banxia Houpu Tang were screened out via TCMSP;CVA-related genes were obtained by GeneCards database,the potential targets of Banxia Houpu Tang in the treatment of CVA were gained after getting the intersection of drug targets with disease targets;network plot of herbs-active ingredients-CVA targets was established by utilizing Cytoscape software;protein-protein interactions(PPI)internet was constructed by using STRING database and Cytoscape software;GO and KEGG of the targets of Banxia Houpu Tang in the treatment of CVA were constructed by using DAVID database.Results:By screening,44 active ingredients and 97 potential targets of Banxia Houpu Tang in the treatment of CVA were gained,141 GO functional entries and 97 KEGG pathways were yielded in enrichment analysis.Conclusion:Banxia Houpu Tang could treat CVA via many ingredients and targets.