LncRNA CCAT1在青少年高血压筛查中的效果及靶向miR-181a促使高血压并发血管疾病的产生

The Effect of LncRNA CCAT1 in the Screening of Adolescent Hypertension and the Targeting of miR-181a Promotes the Occurrence of Hypertension Complicated with Vascular Diseases

目的探究LncRNA CCAT1在青少年高血压筛查中的效果及靶向miR-181a促使高血压并发血管疾病的产生的机制。方法选取山西医科大学汾阳学院附属三甲医院2019年8月-2021年1月收诊高血压患者56例为观察组,同时期健康体检者65例为对照组,检测两组外周血中CCAT1,冠状动脉平滑肌细胞HCASMC、心肌细胞AC16,分析CCAT1、miR-181a的生物学影响情况。最后,双荧光素报告酶验证CCAT1与miR-181a的关系。结果观察组血清中CCAT1高于对照组(P<0.05);ROC曲线分析显示,当血清中CCAT1>2.92时,预测高血压发生的灵敏度为66.07%,特异度为78.46%(P<0.05)。在HCASMC中,sh-CCAT1组增殖能力最强,凋亡率最低,si-CCAT1组增殖能力低于NC-CCAT1组,凋亡率高于NC-CCAT1组(P<0.05);miR-mimics组增殖能力最低,凋亡率最高,miR-inhibitor组增殖能力高于miR-NC组,凋亡率低于miR-NC组(P<0.05)。而在AC16中则反之(P<0.05)。ENCORI发现CCAT1与miR-181a存在可结合的互补位点,双荧光素报告酶显示CCAT1-WT的荧光活性均受到miR-181a模拟物的抑制(P<0.05)。拯救实验则显示过表达CCAT1后对HCASMC与AC16产生的影响完全被升高AC16逆转(P>0.05)。结论CCAT1在青少年高血压中呈高表达,具有成为青少年高血压血液标志物的潜力;CCAT1可通过靶向miR-181a促进HCASMC活化与AC16的凋亡引起冠心病、心力衰竭等疾病的发生。

Objective To explore the role of LncRNA CCAT1 in adolescent hypertension and the mechanism that affects hypertension and vascular disease.Methods Fifty-six patients with hypertension admitted in our hospital from August 2019 to January 2021 were selected as the observation group,and 65 patients with healthy physical examination during the same period were selected as the control group.The CCAT1 in the peripheral blood of the two groups was detected.In addition,human coronary artery smooth muscle cells HCASMC and human cardiomyocyte AC16 were purchased to analyze the biological effects of CCAT1 and miR-181a.Finally,the dual-luciferin reporter enzyme verified the relationship between CCAT1 and miR-181a.Results The serum CCAT1 in the observation group was higher than that in the control group(P<0.05);ROC curve analysis showed that when the serum CCAT1>2.92,the sensitivity of predicting hypertension was 66.07%and the specificity was 78.46%(P<0.05).In HCASMC,the sh-CCAT1 group had the strongest proliferation ability and the lowest apoptotic rate.The si-CCAT1 group had a lower proliferation ability than the NC-CCAT1 group,and the apoptosis rate was higher than that of the NC-CCAT1 group(P<0.05);miR-mimics group The proliferation ability was the lowest and the apoptosis rate was the highest.The proliferation ability of the miR-inhibitor group was higher than that of the miR-NC group,and the apoptosis rate was lower than that of the miR-NC group(P<0.05).The opposite was true in AC16(P<0.05).ENCORI found that CCAT1 and miR-181a can bind to complementary sites,and the dual luciferase reporter enzyme showed that the fluorescence activity of CCAT1-WT was inhibited by miR-181a mimics(P<0.05).Rescue experiments showed that the effects of overexpression of CCAT1 on HCASMC and AC16 were completely reversed by increasing AC16(P>0.05).Conclusion CCAT1 is highly expressed in adolescent hypertension and has the potential to become a blood marker of adolescent hypertension;CCAT1 can promote the activation of HCASMC and the apoptosis of AC16 by targeting miR-181a to cause coronary heart disease,heart failure and other diseases.