血浆外泌体miR-125a-5p对宫颈癌的诊断价值

Diagnostic value of plasma exosome mir-125a-5p in cervical cancer

目的探索血浆外泌体miR-125a-5p对宫颈癌的诊断价值。方法选取2019年5月~2021年10月衡阳市中心医院收治的76例宫颈癌患者,50例正常女性人群(对照组)为研究对象。提取宫颈癌组和对照组血浆外泌体,透射电子显微镜观察外泌体形态,Nano Sight纳米粒度仪检测外泌体颗粒浓度。实时荧光定量PCR(RT-qPCR)检测血浆外泌体miR-125a-5p水平。分析宫颈癌患者miR-125a-5p表达水平与临床病理特征的关系。受试者工作特征(ROC)曲线分析miR-125a-5p诊断宫颈癌的价值。结果宫颈癌组患者的血浆外泌体miR-125a-5p水平低于对照组,差异有统计学意义(P<0.05)。宫颈癌患者血清外泌体miR-125a-5p的表达水平与年龄、病理类型、肿瘤直径、FIGO分期及HPV状态有关(P<0.05)。血清外泌体miR-125a-5p水平预测宫颈癌AUC为0.713(95%CI:0.561~0.865),截断值为1.28,灵敏度和特异度分别为59.1%、84.2%。结论宫颈癌患者血浆外泌体来源的miR-125a-5p表达水平明显降低,对宫颈癌早期诊断具有一定价值。

Objective To evaluate the diagnostic value of plasma exosome miR-125a-5p for cervical cancer.Methods 76 cases cervical cancer patients in our hospital from May 2019 to October 2021 were selected.50 normal women were selected as the control group.The plasma exosomes of two groups were extracted.The morphology of exosomes was observed by transmission electron microscope.The concentration of exosome particles was detected by Nano Sight particle sizer.The level of miR-125a-5p in plasma exosomes was detected by real-time fluorescence quantitative PCR(RTPCR).The relationship between the expression level of miR-125a-5p and clinicopathological features in patients with cervical cancer was analyzed.The diagnostic value of miR-125a-5p for cervical cancer was analyzed by receiver operating characteristic(ROC)curve.Results The level of plasma exosomal miR-125a-5p in the cervical cancer group was lower than that in the control group(P<0.05).The expression level of serum exosomal miR-125a-5p in patients with cervical cancer was related to age,pathological type,tumor diameter,FIGO stage and HPV status(P<0.05).The AUC of cervical cancer predicted by serum exosomal miR-125a-5p level was 0.713(95%CI:0.561~0.865),the cut-off value was 1.28,and the sensitivity and specificity were 59.1%and 84.2%respectively.Conclusion The expression level of miR-125a-5p derived from plasma exosomes in patients with cervical cancer significantly decreases,which is significance for the diagnosis of cervical cancer.