JAK2/STAT3通路在HBV相关肝细胞癌中表达及机制研究

The Expression and Mechanism of JAK2/STAT3 Pathway in HBV-related Hepatocellular Carcinoma

目的探讨JAK2/STAT3通路在HBV相关肝细胞癌中的表达及机制研究。方法选取2019年2月-2021年4月山西省人民医院诊治的HBV相关肝细胞癌患者和非HBV相关肝细胞癌患者各15例。采用Western blot法检测癌组织及癌旁组织JAK2、p-JAK2、STAT3和p-STAT3蛋白的表达,进一步检测稳定表达HBx的HepG2细胞株JAK2、p-JAK2、STAT3和pSTAT3蛋白的表达。结果HBV-HCC组和HCC组癌组织中JAK2、p-JAK2、STAT3、p-STAT3蛋白表达水平均高于癌旁组织,差异有统计学意义(P<0.05);HBV-HCC组较HCC组癌组织中JAK2、STAT3蛋白表达升高,差异无统计学意义(P>0.05),但p-JAK2、p-STAT3均上调,且差异有统计学意义(P<0.05);HBV-HCC组较HCC组及正常对照组JAK2、STAT3磷酸化水平均升高,差异有统计学意义(P<0.05);HepG2-HBx组中JAK2、p-JAK2、STAT3、p-STAT3蛋白表达水平均高于HepG2-vector对照组,差异有统计学意义(P<0.05);HepG2-HBx组较HepG2-vector组JAK2和STAT3磷酸化水平升高,差异有统计学意义(P<0.05)。结论HBV相关肝细胞癌患者HBx蛋白可能通过异常激活JAK2/STAT3信号通路而参与肿瘤的发生发展。

Objective To investigate the expression and mechanism of JAK2/STAT3 pathway in HBV-related hepatocellular carcinoma.Methods From February 2019 to April 2021,15 patients with HBV-related hepatocellular carcinoma and 15 patients with non-HBV-related hepatocellular carcinoma were selected from Shanxi Provincial People’s Hospital.The expression of JAK2,p-JAK2,STAT3 and p-STAT3 protein in cancer tissues and adjacent tissues was detected by Western blot.The expression of JAK2,p-JAK2,STAT3 and p-STAT3 protein in HepG2 cell line stably expressing HBx was further detected.Results The expression levels of JAK2,p-JAK2,STAT3 and p-STAT3 proteins in cancer tissues of HBV-HCC group and HCC group were higher than those in adjacent tissues,and the differences were statistically significant(P<0.05).The expression of JAK2and STAT3 protein in HBV-HCC group was higher than that in HCC group,but the difference was not statistically significant(P>0.05),while p-JAK2and p-STAT3 were up-regulated,the difference was statistically significant(P<0.05).The phosphorylation levels of JAK2 and STAT3 in HBV-HCC group were significantly higher than those in HCC group and normal control group,and the difference was statistically significant(P<0.05).The protein expression levels of JAK2,p-JAK2,STAT3 and p-STAT3 in HepG2-HBx group were higher than those in HepG2-vector control group,and the difference was statistically significant(P<0.05).The phosphorylation levels of JAK2 and STAT3 in HepG2-HBx group were significantly higher than those in HepG2-vector group,and the difference was statistically significant(P<0.05).Conclusion HBx protein may be involved in the occurrence and development of HBV-related hepatocellular carcinoma by abnormally activating JAK2/STAT3 signaling pathway.