消痰散结方联合放疗对肺癌大鼠癌细胞凋亡、免疫功能及肿瘤标志物的影响

Effect of Xiaotan Sanjie Decoction Combined with Radiotherapy on Apoptosis,Immune Function and Tumor Markers of Lung Cancer Cells in Rats

目的观察消痰散结方联合放疗对肺癌大鼠癌细胞凋亡、免疫功能及肿瘤标志物影响。方法选取SD雄性大鼠进行肺癌建模,建模成功后随机分为模型组、消痰散结方组、放疗组、消痰散结方联合放疗组,另设正常组,每组10只。中药组予消痰散结方煎剂0.4 mL/只灌胃,放疗组给予放射治疗,联合组给予消痰散结方联合放疗治疗,观察并记录大鼠体质量、肿瘤质量及肿瘤体积,HE染色法检测组织病理形态,TUNEL法检测癌细胞凋亡,流式细胞仪检测免疫功能相关指标,ELISA法检测肿瘤标志物。结果与正常组相比,模型组体质量、血清CD3+、CD4+细胞含量显著降低(P<0.05),肿瘤质量、肿瘤体积、CD8+、NKT细胞、CEA、NSE、CA125含量显著升高(P<0.05);与模型组相比,中药组体质量、癌细胞凋亡、血清CD3+、CD4+细胞含量明显升高(P<0.05),肿瘤质量、肿瘤体积、CD8+、NKT细胞、CEA、NSE、CA125含量显著降低(P<0.05);与中药组相比,放疗组体质量、肿瘤质量、肿瘤体积、癌细胞凋亡、血清CD3+、CD4+细胞含量、CD8+、NKT细胞含量、CEA、NSE、CA125水平无明显差异(P>0.05);与放疗组和中药组相比,联合组体质量、癌细胞凋亡、血清CD3+、CD4+细胞含量明显升高(P<0.05),肿瘤质量、肿瘤体积、CD8+、NKT细胞、CEA、NSE、CA125含量显著降低(P<0.05)。正常组肺组织结构、肺泡正常完整,排列整齐,间质均匀且无充血;模型组可见肺泡囊明显扩张,肺间质明显增厚,纤毛上皮细胞出现明显破损,可见大量炎性细胞及癌细胞浸润,核仁明显,排列紊乱并伴有腺样或乳头状结构;与模型组比较,中药组、放疗组、联合组病理结构明显改善,炎性细胞及癌细胞明显减少。结论消痰散结方联合放疗可显著促进肺癌大鼠癌细胞凋亡,提高其免疫功能,并降低肿瘤标志物的水平。

Objective:To investigate the effects of Xiaotan Sanjie Decoction combined with radiotherapy on apoptosis,immune function and tumor markers of lung cancer cells in rats.Methods:Lung cancer models was established in SD male rats.After successful modeling,they were randomly divided into model group,Xiaotan Sanjie Decoction group,radiotherapy group,Xiaotan Sanjie Decoction combined with radiotherapy group,and normal group,with 10 rats in each group.Xiaotan Sanjie Decoction group was given 0.4 mL of Xiaotan Sanjie Decoction,radiotherapy group was given radiotherapy,and combination group was given Xiaotan Sanjie Decoction combined with radiotherapy.The body mass,tumor mass and tumor volume of rats were observed and recorded,Histopathological morphology was detected by HE staining,apoptosis of cancer cells was detected by TUNEL,immune function related indicators were detected by flow cytometry,and tumor markers were detected by ELISA.Results:Compared with normal group,the body weight,serum contents of CD3+and CD4+cells in model group were significantly decreased(P<0.05),tumor weight,tumor volume,contents of CD8+,NKT cells,CEA,NSE and CA125were significantly increased(P<0.05).Compared with model group,the body weight,apoptosis of cancer cells,serum contents of CD3+and CD4+cells in Xiaotan Sanjie Decoction group were significantly increased(P<0.05),tumor weight,tumor volume,contents of CD8+,NKT cells,CEA,NSE and CA125 were significantly decreased(P<0.05).Compared with Xiaotan Sanjie Decoction group,there were no significant differences in body weight,tumor weight,tumor volume,cell apoptosis,serum contents of CD3+,CD4+cell content,CD8+,NKT cells,CEA,NSE and CA125 levels in radiotherapy group(P>0.05).Compared with radiotherapy group,the body weight,apoptosis of cancer cells,serum contents of CD3+and CD4+cells in combination group were significantly increased(P<0.05),while tumor weight,tumor volume,serum contents of CD8+,NKT cells,CEA,NSE and CA125 were significantly decreased(P<0.05).In normal group,the structure of the lung tissue and alveoli were normal and intact,arranged in an orderly fashion,the interstitium was even and without congestion;in model group,the alveolar sac was obviously dilated,the lung interstitium was obviously thickened,the ciliated epithelial cells appeared obviously broken,a large number of inflammatory cells and cancer cell infiltration were visible,the nucleolus was obvious,arranged in a disorder and accompanied by adenoid or papillary structure.Compared with model group,the pathological structure of Xiaotan Sanjie Decoction groups,radiotherapy groups,and Xiaotan Sanjie Decoction combined with radiotherapy group was significantly improved,and the inflammatory cells and cancer cells were obviously reduced.Conclusion:Xiaotan Sanjie Decoction combined with radiotherapy can significantly promote the apoptosis of lung cancer cells in rats,improve the immune function,and reduce the level of tumor markers.