食品中椰毒假单胞菌微滴式数字PCR定量检测方法的建立

Establishment of Droplet Digital PCR Assay for Quantitative Detection of Pseudomonas cocovenenans in Foods

椰毒假单胞菌可污染包括发酵米面等多种食物,其产生的两种毒素导致严重的食物中毒,威胁人体健康。基于数字PCR技术,建立了针对椰毒假单胞菌的定量测量方法。该方法具有较好的特异性与重复性,人工污染糯米汤样品中的检出限为361 CFU/mL。与平板培养法相比,基于数字PCR技术的测量方法缩短了测量时间,有利于椰毒假单胞菌的快速准确测量,未来可在食品安全监测与中毒分析等领域进行应用。

Pseudomonas cocovenenans contaminates a variety of foods including fermented rice and noodles.It produces two toxins that cause severe food poisoning,which threatens human health.Based on digital PCR technology,the quantitative method targeting to P.cocovenenans was established.It demonstrated good specificity and repeatability.The detection limit in polluted glutinous rice soup was 361 CFU/mL.Compared with plate counting method,the detection time was shortened obviously via the digital PCR quantitative method,which was conducive to the rapid and accurate measurement of P.cocovenenans.Thus,it may be applied in the fields of food safety monitoring and poisoning analysis in the future.