基于网络药理学的制天南星炮制增效研究

Study on processing and enhance the curative effects of Arisaematis rhizomz preparatum based on network pharmacology

目的探讨制天南星炮制增效机理。方法采用UPLC-Q-TOF-MS/MS技术检测天南星炮制前后的化学成分,应用网络药理学分析其治疗“喘、痰”的作用机制,建立过敏性哮喘大鼠模型对天南星炮制前后药效进行比较。结果制天南星中共鉴定出27个化学成分,其中姜黄素、6-姜烯酚等成分是炮制后增加的。结合数据库预测共通过网络药理学探讨预测了36个天南星中的化学成分治疗“喘、痰”疾病过程中的作用机制,动物实验结果表明,制天南星治疗过敏性哮喘的作用优于生品,但差异无统计学意义。结论天南星炮制后增加了姜黄素、6-姜烯酚、樟脑、去甲基姜黄素等成分,可能是制天南星治疗过敏性哮喘疗效增效的原因。

Aim To explore the mechanism of processing and increasing efficiency of Arisaematis rhizomz preparatum.Methods UPLC-Q-TOF-MS/MS technology was used to detect the chemical components before and after processing of Arisaematis rhizomz preparatum,and its mechanism of action was analysed in the treatment of“asthma and phlegm”by using network pharmacology.A rat model of allergic asthma was established to compare the efficacy of Arisaematis rhizoma before and after processing.Results A total of 27 chemical components were identified,among which curcumin,6-gingerol and other components increased after processing.Combined with the database prediction,the action mechanism of the 36 chemical components in the treatment of“asthma and phlegm”diseases was dis-cussed and predicted through network pharmacology.The results of animal experiments showed that the effect of processed Arisaematis rhizoma on allergic asthma was better than that of Arisaematis rhizoma,but there was no significant difference.Conclusions The addition of curcumin,6-gingerol,camphor,demethylcurcumin and other components after the processed Arisaematis rhizomz preparatum may be the reason for the synergistic effect of Arisaematis rhizomz preparatum in the treatment of allergic asthma.