广东沿海养殖牡蛎中副溶血性弧菌的污染状况、毒力基因及耐药性分析

Analysis of contamination,virulence genes and antimicrobial resistance of Vibrio parahaemolyticus in cultured oysters from the coast in Guangdong province

对广东沿海养殖牡蛎中副溶血性弧菌的污染状况、毒力基因携带情况和耐药特征进行研究。结果显示,320份牡蛎中副溶血性弧菌检出率为18.8%,不同季节和不同地区差异显著;分离得到60株副溶血性弧菌,均检出tlh基因,未检出tdh和trh基因;26种药敏分析发现,分离株普遍对氨苄西林耐药,耐药率为83.3%,对哌拉西林、四环素和呋喃妥因等8种抗生素的耐药率介于1.67%~11.7%。所有菌株对头孢泊肟、亚胺培南、阿米卡星等12种抗生素敏感;分离株的多重耐药率为3.33%,其中1株菌出现耐受7种抗生素的多重耐药。结果表明,广东沿海养殖牡蛎的副溶血性弧菌污染程度在不同地区及季节存在显著差异,分离株的致病风险较低并呈现不同程度的耐药性,其中惠州地区养殖牡蛎中副溶血性弧菌污染及耐药程度相对较高。

To investigate the contamination,virulence gene and antimicrobial resistance of Vibrio parahaemolyticus in cultured oysters from the main culture areas along the coast of Guangdong.The results showed that the average detection rate of Vibrio parahaemolyticus was 18.8%among 320 oyster samples.There were significant differences of incidence among different seasons and regions.60 strains were isolated from the positive samples.All the isolates carried the tlh gene,while the tdh and trh gene were not detected in all isolates.26 kind of antibiotics were used for drug sensitivity test.The results revealed that most of the isolates were generally resistant to ampicillin,and the resistance rate was 83.3%.Some strains were resistant to 8antibiotics,such as piperacillin,tetracycline and nitrofurantoin,etc,and the resistance rates were1.67%~11.7%.All the isolates were sensitive to 12 antibiotics,such as cefpodoxime,imipenem,amikacin,etc.The multi-drug resistance rate of the isolates was 3.33%,and a strain showed multi-drug resistance to 7antibiotics.The results indicated that there were significant differences of Vibrio parahaemolyticus contamination level among different seasons and regions in cultured oysters along the coast of Guangdong.The isolates had a low risk of disease and showed different degrees of drug resistance.Among the oysters farming areas,Huizhou showed a higher contamination rate and resistance degree than other areas.