基于DNA分子标记的类烟产品鉴别检验抽样方法研究

Study on Sampling Method for Identification and Inspection of Cigarette-like Product Based on DNA Molecular Marker

[目的]探索高效、准确地鉴别类烟产品的抽样方案和检验方法,对保障真品卷烟的合理流通、市场监管和烟草专卖具有重要意义。[方法]以400支类烟产品(茶烟)为材料,按统计抽样检验原理,研究设计适合类烟产品最小抽样量和最大抽样量的序贯抽样检验方案,以卷烟标准样品为参比,利用已开发的DNA标记对抽取的类烟产品准确性、可靠性进行鉴定验证。[结果]该研究建立的序贯抽样检验方案保证了目前行业标准检验方法的可靠性和精确度,使用该方案,确定了抽样理论最小样本数52个,最大抽样量363个,最多进行11次检验即可得出鉴别结论,且抽样特性曲线具有相对较高的稳定性和灵敏度。DNA标记检验表明,卷烟标准样品在250 bp分子量处扩增出了特异性条带,而初次抽样的52个类烟产品均未在250 bp分子量处扩增出特异性条带。[结论]按该研究设计的序贯抽样检验方法,只需检验很小的样本(52个),经DNA标记验证,其抽样确定的类烟产品真伪准确、客观。

[Objective]To explore the sampling plan and inspection method for efficient and accurate identification of cigarette-like product,which is of great significance for ensuring the reasonable circulation,market supervision and tobacco monopoly of authentic cigarettes.[Method]Taking 400 cigarette-like product as test material,the experiment was designed a sequential sample inspectial plan about minimum and maximum sample quantity according to the principle of statistical,and DNA molecular marker technology had been used to identification accuracy,reliability of cigarette-like product containing tobacco ingredient.[Result]The sequential sampling method was established to ensure the reliability and the accuracy of industry standard test methods.This sampling plan possessed a minimum sample size of 52,a maximum sample size of 363,and needed 11 rounds at most to draw the inspection conclusion.The determined sampling characteristic curve had relatively high certainty and sensitivity.The specific bands were amplified at 250 bp molecular weight in the cigarette standard samples,but not at 250 bp molecular weight in the 52 cigarette-like products.[Conclusion]According to the sequential sampling inspection method,only a very small tested sample could be required by 52 cigarettes.Meanwhile,the sample of cigarette-like product are accurate and objective through DNA marker verification.