核酸试纸条检测方法研究进展

Progress of Nucleic Acid Detection Strip Detection Approaches

凝胶电泳、实时荧光PCR等常规核酸检测方法存在操作繁琐、设备昂贵、反应时间长等局限性。随着核酸检测市场规模的大幅提升,常规检测方法已无法满足临床诊断、检验检疫的需求。核酸试纸条(nucleic acid detection strip,NADS)是一种新兴的核酸检测方法,具有灵敏度高、操作便捷、结果可视化、成本低且耗时短等优势,在基础研究与临床诊断等领域受到广泛关注。综述近年来NADS的检测方法及研究进展,系统总结该技术的原理、应用及临床潜在转化价值,以期为NADS的进一步开发、利用提供借鉴。

With the growth of the nucleic acid testing market,conventional nucleic acid detection approaches such as gel electrophoresis and quantitative real-time PCR(qPCR)have gradually failed to meet the needs of clinical analysis,inspection and quarantine due to their limitations(cumbersome operation,expensive equipment,long reaction time and so on).Nucleic acid detection strip(NADS),a new nucleic acid detection approach with the advantages of high sensitivity,convenient operation,visualization of results,low cost and short time consuming,has attracted extensive attention in basic research and clinical diagnosis.The new progress of NADS in the past ten years was systematically introduced.Meanwhile,the principle,advantages and clinical potential translation value of NADS were summarized in order to provide reference for the further development and utilization of nucleic acid detection strip.