链霉菌中3个新脂肪酰胺类化合物

Three new acylamide compounds from Streptomyces plumbidurans

特殊生境放线菌具有较大的开发前景,因其独特的生存环境可以产生新颖且具有生物活性的次生代谢产物.研究Streptomyces plumbidurans发酵液的次生代谢成分,并寻找具有抗肿瘤及抗菌活性的小分子化合物.利用凝胶Sephadex LH-20柱色谱、D101大孔树脂柱色谱、硅胶柱色谱等分离技术,并采用现代波谱学方法鉴定化合物的结构.采用噻唑蓝比色法测试化合物1~3抗肿瘤细胞毒活性;采用微量稀释法检测体外抑菌活性.从Streptomyces plumbidurans发酵液中确定3个新脂肪酰胺类化合物,并依次命名为:kcacylamide A(1)、kcacylamide B(2)和kcacylamide C(3),生物活性结果显示3个化合物在50μmol/L均无明显抗肿瘤细胞毒活性及抑菌活性.研究首次发现Streptomyces plumbidurans会产生脂肪酰胺类化学物质.

Special habitat actinomycetes have great development prospects because their unique living environment can produce novel and biologically active secondary metabolites.The secondary metabolites of Streptomyces plumbidurans were studied,and small molecular compounds with antitumor and antimicrobial activities were searched.The secondary metabolites were isolated by Sephadex LH-20 column chromatography,D101 macroporous resin,and RP-18 reverse-phase column chromatography from the EtOAc extract of the culture of Streptomyces plumbidurans.The structures were characterized by various spectroscopic(1H NMR,13C NMR and MS)and comparison with the data of literatures.MTT method and microdilution method were used to test the antitumor activity in vitro and antimicrobial activity of the above compounds.Three new compounds identified as kcacylamide A(1),kcacylamide B(2)and kcacylamide C(3).Compounds 1-3 did not show significant activity at the concentration of 50μmol/L.In this study,we found that Streptomyces plumbidurans could produce acylamide compounds for the first time.