抗生素对硝化污泥系统的影响及其去除特性

Effect of Antibiotics on Nitrification Sludge System and the Removal Characteristics

硝化污泥系统能够有效去除多种抗生素,然而抗生素对硝化污泥系统运行性能和微生物群落结构的影响仍不明确。采用硝化污泥系统降解抗生素磺胺甲恶唑(SMX),考察系统中不同质量浓度SMX(0.1、1.0、10.0 mg/L)的去除效果,研究SMX对硝化污泥系统运行性能及微生物群落结构的影响。结果显示,不同质量浓度的SMX(0.1、1.0、10.0 mg/L)去除率分别为93.60%、91.54%、89.22%,硝化污泥系统能够较好地适应SMX的压力冲击,不同SMX浓度条件下化学需氧量(CODCr)和氨氮的去除率均高于90%。16S rRNA高通量测序结果表明,SMX浓度越高,对微生物群落结构影响越大,0.1 mg/L和1.0 mg/L SMX的去除是氨氧化细菌[亚硝化单胞菌属(Nitrosomonas)、unidentified_Nitrospiraceae]和优势菌[球衣菌属(Sphaerotilus)、动胶菌属(Zoogloea)、噬氢菌属(Hydrogenophaga)]共同作用的结果,而10.0 mg/L SMX的高效去除可归因于陶厄氏菌属(Thauera)、固氮弧菌属(Azoarcus)、黄杆菌属(Flavobacterium)的富集。

Nitrification sludge system could effectively remove a variety of antibiotics,but the effect of antibiotics on operation performance and microbial community structure of nitrification sludge system was still unclear.Nitrification sludge system was used to degrade antibiotics sulfamethoxazole(SMX).The removal of different concentrations of SMX(0.1,1.0,10.0 mg/L)in the system was investigated,and the effects of SMX on the operating performance and microbial community structure of nitrification sludge system were studied.The results showed that the removal efficiency of SMX at different concentrations(0.1,1.0 mg/L and 10.0 mg/L)were 93.60%,91.54%and 89.22%,respectively.The nitrification sludge system could better adapt to the impact of SMX pressure.Under different SMX concentrations,the removal rates of chemical oxygen demand(CODCr)and ammonia nitrogen were higher than 90%.16S rRNA high-throughput sequencing results showed that the higher SMX concentration,the greater its impact on the microbial community structure.The removal of 0.1 mg/L and 1.0 mg/L SMX was the result of the joint action of ammonia oxidizing bacteria(Nitrosomonas,Nitrospiraceae)and dominant bacteria(Sphaerotilus,Zoogloea and Hydrogenophaga),while the efficient removal of 10.0 mg/L SMX could be attributed to the enrichment of Thauera,Azoarcus and Flavobacterium.