人参果汁浓缩液对免疫低下小鼠免疫功能的影响

Effects of Panax ginseng Fruit Juice Concentrate on Immune Function in Immunocompromised Mice

目的:研究人参果汁浓缩液对免疫低下小鼠免疫功能的影响。方法:72只雄性SPF级C57BL/6J小鼠随机分成空白组、模型组、人参果汁浓缩液(83、166、249、332 mg/kg m_(b))组,每组12只。模型组和人参果汁浓缩液各剂量组自第1天开始,每日腹腔注射40 mg/kg m_(b)环磷酰胺,连续10 d,空白组腹腔注射等量的磷酸盐缓冲液;同时自第1天开始,人参果汁浓缩液各剂量组每日给予小鼠灌胃20 mL/kg m_(b)不同剂量人参果汁浓缩液,空白组和模型组灌胃等量的蒸馏水,连续15 d后测定小鼠体质量、脾脏和胸腺质量、白细胞数量、淋巴细胞亚群数量、细胞因子表达水平以及免疫细胞核转录因子表达水平。结果:人参果汁浓缩液可改善免疫低下小鼠的脾脏质量/指数,提高免疫低下小鼠外周血细胞数量和免疫器官中淋巴细胞亚群数量,促进免疫低下小鼠细胞因子白细胞介素(interleukin,IL)-2、IL-4和IL-17的表达和抑制负调控因子转化生长因子(transforming growth factor,TGF)-β的表达,以及部分恢复免疫细胞核转录因子T细胞中T-box(T-box expressed in T cells,T-bet)和叉头样转录因子3(forkhead box protein 3,Foxp3)mRNA的表达(P<0.05);其中,以249 mg/kg m_(b)人参果汁浓缩液效果最为显著;人参果汁浓缩液对免疫低下小鼠体质量和胸腺质量的恢复无明显效果。结论:人参果汁浓缩液可以增强免疫低下小鼠的免疫功能。

Objective:To explore the effects of Panax ginseng fruit juice concentrate on immune function in immunocompromised mice.Methods:Totally 72 specific pathogen-free(SPF)C57BL/6J male mice were randomly divided into six groups(n=12):blank,model,and low-,medium-and high-dose Panax ginseng fruit juice concentrate(83,166,249 and 332 mg/kg m_(b)).The model and Panax ginseng fruit juice concentrate groups were injected intraperitoneally with 40 mg/kg m_(b)cyclophosphamide on a daily basis for 10 days while the blank group was intraperitoneally injected with the same amount of phosphate buffered saline(PBS).Afterwards,the mice in the Panax ginseng fruit juice concentrate groups were intragastrically administrated with different dosage of Panax ginseng fruit juice concentrate at a volume of 20 mL/kg m_(b),and those in the blank and model groups were gavaged with the same volume of distilled water for 15 days.Then,body,spleen and thymus mass,white blood cell count,the number of lymphocyte subsets,cytokine expression levels,and nuclear transcription factor expression levels in immune cells were measured.Results:Panax ginseng fruit juice concentrate could improve the spleen mass/index(spleen/body mass ratio)in immunocompromised mice,increase the number of peripheral blood cells and the number of lymphocytes in immune organs,promote the expression of cytokines interleukin(IL)-2,IL-4 and IL-17,inhibit the expression of transforming growth factor(TGF)-βin immunocompromised mice and partially restore the RNA expression of T-box expressed in T cells(T-bet)and forkhead box protein 3(Foxp3)(P<0.05),and the effect of Panax ginseng fruit juice concentrate at a dose of 249 mg/kg m_(b)was the most significant.However,Panax ginseng fruit juice concentrate had no significant effect on restoring the body mass or thymus mass of immunocompromised mice.Conclusion:Panax ginseng fruit juice concentrate can enhance immune function in immunocompromised mice.