酵母甘露糖蛋白体外发酵及其代谢产物的抗炎活性

Fermentation of Yeast Mannoprotein by Intestinal Microorganisms in Vitro and Anti-inflammatory Activity of Its Metabolites

通过体外肠道菌群厌氧发酵技术研究酵母甘露糖蛋白(mannoprotein,MP)的益生活性。结果表明,MP可调节肠道菌群结构,在门水平增加了拟杆菌门(Bacteroidetes)的相对丰度,降低了厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)的相对丰度,降低了Firmicutes/Bacteroidetes比值。MP显著提高了发酵液中短链脂肪酸尤其是乙酸和丙酸的含量。MP与菊粉具有相似的益生菌活性,菊粉可促进双歧杆菌属(Bifidobacterium)的增殖,而MP可选择性促进拟杆菌属(Bacteroides)、韦荣球菌属(Veillonella)、Clostridium_sensu_srticto、布劳特氏菌属(Blautia)、粪杆菌属(Faecalibacterium)、纺锤链杆属(Fusicatenibacter)和产丁酸球菌属(Butyricicoccus)的生长。脂多糖诱导RAW 264.7巨噬细胞模型结果表明MP对炎症没有显著调节作用(P>0.05),而其发酵液具有显著抗炎活性,且其活性与菊粉组相似,均优于空白组。因此,MP有望成为通过调节肠道菌群改善人体健康的功能性食品。

The anaerobic fermentation of yeast mannoprotein(MP)by the gut microbiota in vitro was carried out to evaluate the probiotic activity of MP in this study.The results showed that MP could modulate the structure of the gut microbiota,increasing the relative abundance of Bacteroidetes and decreasing the relative abundances of Firmicutes and Proteobacteria and the ratio of Firmicutes to Bacteroidetes.The concentration of short-chain fatty acids,especially acetic acid and propionic acid,in the fermentation broth were significantly increased by MP.MP showed similar prebiotic activity to inulin.Inulin could stimulate the proliferation of Bifidobacterium,whereas MP could selectively promote the growth of Bacteroides,Veillonella,Clostridium_sensu_srticto,Blautia,Faecalibacterium,Fusicatenibacter and Butyricicoccus.MP had no significant inhibitory effect on lipopolysaccharide(LPS)-induced inflammation in RAW 264.7 macrophages(P>0.05),whereas the fermentation broth showed anti-inflammatory activity similar to that of inulin,which was superior to that of the blank group.Thus,MP is expected to serve as a functional food ingredient that can offer health benefits by modulating the gut microbiota.