蓖麻SAUR基因家族的鉴定及生物信息学分析

Identification and Bioinformatics Analysis of SAUR Gene Family in Castor Bean(Ricinus communis L.)

SAUR作为对生长素响应最强烈的基因之一,在调节植物的细胞膨胀、参与植物的庇荫反应和植物的高温诱导生长等方面扮演着重要的角色。本研究基于蓖麻(大戟科)全基因组数据信息,利用生物信息学手段对蓖麻SAUR基因家族进行鉴定,并分析其家族成员的基础信息。结果表明,在蓖麻中共有35个SAUR家族基因,这些基因不均匀地分布在Chr.1~Chr.9上,并且有42.8%的基因是成簇分布的,基础理化性质分析发现其中31个RcSAUR蛋白是疏水性蛋白,亚细胞预测结果显示分别有12个和11个RcSAUR蛋白定位在线粒体和叶绿体上;物种内进化树分析结果表明,35个RcSAUR基因可被分为3组,且每组基因数量不同;物种间进化树显示,其中7组中均有RcSAUR蛋白,被聚为一类的蛋白在功能上可能具有相似性;蓖麻与拟南芥的种间共线性分析发现,二者的SAUR基因发生了大规模的基因重复事件;另外,RcSAUR家族基因还拥有多个激素响应元件与逆境胁迫作用元件;该家族基因在蓖麻的不同发育阶段的表达量不同,主要在叶片中集中表达。本研究通过对蓖麻SAUR基因家族的信息挖掘,以期对蓖麻分子育种领域提供合理的数据支撑。

As one of the genes most responsive to auxin,SAUR plays an important role in regulating plant cell expansion,participating in plant shading response and plant high temperature-induced growth.In this study,based on the whole genome data of castor(Euphorbiaceae),the SAUR gene family of castor was identified by bioinformatics methods,and the basic information of its family genes was analyzed.The results showed that there were 35SAUR family genes in castor,and these genes were unevenly distributed in Chr.1~Chr.9,and 42.8%of the genes were clustered,based on the analysis of physical and chemical properties,it was found that 31 RcSAUR proteins were hydrophobic proteins.Subcellular prediction results showed that 12 and 11 RcSAUR proteins were located on mitochondria and chloroplasts,respectively;the phyl ogenetic tree analysis showed that 35 RcSAUR genes could be divided into three groups,and the number of genes in each group was different;the phylogenetic tree among species showed that there were RcSAUR proteins in all seven groups,and the proteins clustered into one group might have functional similarities;the analysis of interspecific collinearity between Ricinus communis and Arabidopsis thaliana showed that the SAUR genes had large-scale gene duplication events;in addition,RcSAUR family genes also have multiple hormone response elements and stress response elements.The expression levels of the family genes were different in different developmental stages of castor,mainly concentrated in leaves.In this study,the information of castor SAUR gene family was mined to provide reasonable data support for castor molecular breeding.