血浆B细胞趋化因子1和干细胞因子水平与HBV感染患者临床指标的关系

Correlation of serum B cell-attracting chemokine 1 and stem cell factor with clinical indices of HBV infected patients

目的乙型肝炎病毒(HBV)感染者肝脏炎性反应状态与病毒感染指标的相关性。方法收集HBV感染者及健康者共198例。HBV感染者按谷丙转氨酶/谷草转氨酶(ALT/AST)、乙肝病毒脱氧核糖核酸(HBV-DNA)、乙肝病毒E抗原(HBeAg)及肝硬化等指标进行分组;ELISA检测外周血B细胞趋化因子1(BCA-1)和干细胞因子(SCF)水平;比较BCA-1和SCF在健康者组、不同HBV感染者分组中的差异,并分析其与ALT/AST、HBV-DNA、HBeAg间的相关性;符合正态分布组采用独立样本t检验,非正态分布组采用Mann-Whitney U检验,相关性分析采用Spearman分析。结果HBV感染者血清的BCA-1和SCF水平均显著高于对照组(P<0.001)。HBV感染合并肝硬化患者SCF水平显著高于HBV感染(无肝硬化)者(P<0.01)。BCA-1水平在HBV-DNA(+/-)及甲胎蛋白(AFP)(正常/异常)组间均存在差异(P<0.05),HBV-DNA阳性患者BCA-1水平高于HBV-DNA阴性患者,AFP异常患者BCA-1水平显著低于AFP正常患者。BCA-1与HBV-DNA、HBeAg均存在正相关(R=0.314;R=0.456;P<0.01)。结论HBV感染者外周血BCA-1与HBV DNA水平紧密相关,SCF水平在HBV感染肝硬化患者中明显升高,可能具有潜在的临床诊断价值。

Objective To explore potential correlation between liver inflammatory response and viral infection index in patients with hepatitis B virus(HBV).Methods Totally 198 serum samples from HBV infected patients and healthy subjects were collected.Patients were divided into different groups according to alanine transaminase/aspartate transaminase(ALT/AST),HBV-DNA,hepatitis B E Antigen(HBeAg)and liver cirrhosis situation;ELISA was used to determine the B cell-attracting chemokine 1(BCA-1)and stem cell factor(SCF)level;the relationship between BCA-1,SCF and other clinical indicators,including ALT/AST,HBV-DNA,HBeAg and cirrhosis condition,were analyzed in different groups;the data was analyzed either with unpaired t test,or with Mann-Whitney U test according to normal distribution,and Spearman method was used for correlation analysis.Results The levels of serum BCA-1 and SCF in HBV infected patients were significantly higher than those in healthy controls(P<0.001).The serum SCF level was significantly higher in cirrhosis patients than patients without cirrhosis(P<0.01).The BCA-1 level was significantly different between HBV-DNA+and HBV-patients,as well as between alpha-fetoprotein(AFP)normal and abnormal patients(P<0.05).BCA-1 was significantly higher in HBV-DNA+patients than that in HBV-DNA-patients and was lower in AFP abnormal patients than in normal patients.Furthermore,BCA-1 was positively relate to HBV-DNA and HBeAg level(R=0.314,R=0.456,P<0.01).Conclusions BCA-1 was significantly related to HBV DNA,while SCF is upregulated in cirrhosis patients with HBV infection,which may be the potential candidate of clinical indicators for HBV infection.