反相高效液相色谱法测定土壤中多环芳烃

Determination of polycyclic aromatic hydrocarbons in soil by reversed phase high performance liquid chromatography method

建立了一种同时测定土壤中16种多环芳烃的反相高效液相色谱分析法。以乙腈为流动相,结合索氏提取和梯度洗脱,实现了16种多环芳烃在C_(18)色谱柱上的有效分离。考察了波长、梯度洗脱模式、流速、柱温等对分离效果的影响,结果表明,在最优化的实验条件下,16种多环芳烃分离效果良好,分离度在1.00~8.90范围内,方法检出限在3~5μg/kg范围内,平均加标回收率在82.4%~105%范围内,相对标准偏差在3.2%~8.3%范围内。反相高效液相色谱分析法已成功应用于环保企业土壤样品中多环芳烃的分析,达到了满意的效果。

A method for simultaneous determination of 16 kinds of polycyclic aromatic hydrocarbons(PAHs)in soil by reversed phase high performance liquid chromatography was established.Using acetonitrile as mobile phase,combined with soxhlet extraction and gradient elution,16 kinds of PAHs were effectively separated on C_(18)column.The effects of wavelength,gradient elution mode,flow rate and column temperature on the separation efficiency were investigated,the results showed that under the optimal experimental conditions,the separation effect of 16 kinds of PAHs were separated effectively,the resolution was in the range of 1.00~8.90,the detection limit of the method was in the range of 3~5μg/kg,the average spiking recovery rate was in the range of 82.4%~105%,and the relative standard deviation was in the range of 3.2%~8.3%.Reverse phase high performance liquid chromatography(RP-HPLC)has been successfully applied to the analysis of PAHs in soil samples of environmental protection enterprises,and achieved satisfactory results.