补喂L-精氨酸对返情纯血母马妊娠率及血浆参数的影响

Effect of Supplementation with L-arginine on Pregnancy Rate and Plasma Parameters of Re-service Thoroughbred Horses

【目的】试验旨在研究补喂L-精氨酸对返情纯血马妊娠率及血液参数的影响。【方法】选择返情纯血母马14匹随机分为2组,每组7匹(n=7),分别为对照组、L-精氨酸组,对照组饲喂基础饲粮,L-精氨酸组在基础饲粮中添加50 g/d L-精氨酸;预饲期7 d,试验期30 d。返情母马发情时,第一次人工输精结束当天补喂L-精氨酸;配种结束时直肠B超检查妊娠情况,统计妊娠率,分别于试验第0、15、30天颈静脉采血,用于测定总一氧化氮合成酶(total nitric oxide synthetase,TNOS)、内皮型一氧化氮合成酶(endothelial nitric oxide synthetase,eNOS)活性及一氧化氮(nitric oxide,NO)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、生长激素(growth hormone,GH)、胰岛素样生长因子(insulinlike growth factor,IGF)含量等指标。【结果】与对照组相比,L-精氨酸组妊娠率显著提高(P<0.05);试验第0、15、30天血浆TNOS、eNOS、鸟氨酸脱羧酶(orinithine decarboxylase,ODC)活性及NO、IGF-Ⅰ、IGF-Ⅱ、VEGF含量在两组间均无显著差异(P>0.05);试验第30天L-精氨酸组血浆氨(ammonia,NH3)、尿素氮(urea nitrogen,UN)含量分别比对照组降低13.49%、26.91%(P<0.05)。与对照组相比,试验第30天L-精氨酸组血浆GH含量提高50.26%(P<0.01),试验第0、15、30天两组间母马血浆免疫球蛋白A(IgA)、免疫球蛋白M(IgM)、免疫球蛋白G(IgG)含量均无显著差异(P>0.05)。【结论】返情纯血母马补喂L-精氨酸能显著提高妊娠率,改变母马体循环血浆UN、NH3、GH含量。L-精氨酸可作为营养调控剂提高纯血母马妊娠率。

【Objective】 The purpose of the experiment was to study the effect of supplementation with L-arginine on pregnancy rate and plasma parameters of re-service thoroughbred horses.【Method】 14 re-service thoroughbred horses were randomly divided into 2 groups, 7 in each group(n=7),as the control group and L-arginine group.The control group was fed with basic diet, and the L-arginine group was added with 50 g/d L-arginine on the basis of basic diet.The pre feeding period was 7 d, and the trial period was 30 d.When the returning mare was in estrus, L-arginine was supplemented on the day after the first artificial insemination.At the end of mating, the pregnancy was examined by rectum B-ultrasound, and the pregnancy rate was calculated.Blood was collected from the jugular vein of mares at 0, 15 and 30 d respectively.The activities of total nitric oxide synthase(TNOS),endothelial nitric oxide synthase(eNOS) and the contonts of nitric oxide(NO),vascular endothelial growth factor(VEGF),growth hormone(GH),insulin-like growth factor(IGF) were measured.【Result】 Compared with the control group, the pregnancy rate of the L-arginine group was significantly increased(P<0.05).There was no significant difference in plasma TNOS,eNOS,NO,ODC,IGF-Ⅰ,IGF-Ⅱ and VEGF levels/activities between the two groups at 0,15 and 30 d(P>0.05).On the 30th day of the test, the plasma ammonia(NH3) and urea nitrogen(UN) contents in the L-arginine group decreased by 13.49% and 26.91% respectively compared with the control group(P<0.05).Compared with the control group, the plasma GH content on the 30th day of the test was significantly increased by 50.26% in L-arginine group(P<0.01).And there was no significant difference in the contents of IgA,IgM and IgG of mares on the 0th, 15th, and 30th days of the test between two groups(P>0.05).【Conclusion】 Feeding L-arginine to the re-service thoroughbred horses could significantly increase the pregnancy rate and change the contents of UN,NH3 and GH in the circulating plasma of the mares.L-arginine could be used as a nutritional regulator to improve the pregnancy rate of thoroughbred mares.