呋喃酚葡萄糖醛酸结合物在呋喃丹染毒家兔体内的死后分布及死后再分布

Postmortem Distribution and Postmortem Redistribution of Carbofuran-7-Phenyl Glucuronic Acid in Rabbits

目的建立呋喃丹的家兔灌胃染毒致死模型,观察呋喃丹Ⅱ相代谢物呋喃酚葡萄糖醛酸结合物(carbofuran-7-phenyl glucuronic acid,Glu-7PH)在家兔体内的死后分布及死后再分布规律。方法死后分布:对家兔使用1/2LD50、LD50、2LD50呋喃丹灌胃染毒,染毒死亡家兔立即解剖,灌胃后2 h仍未死亡家兔采用二氧化碳(CO_(2))吸入法处死后立即解剖,取心肌、心血、肝、脾、肺、肾、脑和右下肢肌肉。死后再分布:家兔用4LD50呋喃丹灌胃染毒,仰卧位置于室温(15℃)条件下,分别于死后0、12、24、48、72 h取心肌、心血、肝、脾、肺、肾、脑和右下肢肌肉。采用液相色谱-串联质谱(liquid chromatography-tandem mass spec⁃trometry,LC-MS/MS)法检测Glu-7PH含量。结果死后分布结果显示,3个剂量组中,各组织中Glu-7PH含量差异存在统计学意义。死后再分布研究结果显示,心血、心肌、脾、肾、脑及右下肢肌肉中Glu-7PH含量差异无统计学意义,肝、肺中Glu-7PH含量差异存在统计学意义。结论家兔心肌、心血、肝、肺、肾、脑和下肢肌肉可作为Glu-7PH检测分析样本。Glu-7PH在家兔肝和肺中存在死后再分布现象。

Objective To establish a carbofuran intragastric administration death model in rabbits,and to observe the postmortem distribution and postmortem redistribution of carbofuran-7-phenyl glucuronic acid(Glu-7PH)in rabbits.Methods The postmortem distribution:Rabbits were given an administration of 1/2LD50,LD50,2LD50 carbofuran.Dead rabbits were dissected immediately.Rabbits that had remained alive 2 hours were sacrificed by carbon dioxide(CO_(2))inhalation and dissected immediately.The myocardium,cardiac blood,liver,spleen,lung,kidney,brain and right hindlimb muscle were collected.The postmortem redistribution:After giving an administration of 4LD50 carbofuran,the myocardium,cardiac blood,liver,spleen,lung,kidney,brain,and right hindlimb muscle were collected at 0,12,24,48,and 72 h postmortem in supine position at 15℃room temperature.The quantity of Glu-7PH was determined by LC-MS/MS.Results The postmortem distribution:Among the three dose groups,there were significant differences in the quantities of Glu-7PH in different tissues.The postmortem redistribution:There was no significant difference in the Glu-7PH quantities in cardiac blood,mycardium,spleen,kidney,brain and right hindlimb muscle,but there was a significant difference in the Glu-7PH quantities in the liver and lung.Conclusion The mycardium,cardiac blood,liver,lung,kidney,brain and hindlimb muscle of rabbits can be used as appropriate samples for Glu-7PH detection.However,it should be noted that Glu-7PH was redistributed postmortem in rabbit liver and lung.