福建三沙湾海域细菌群落结构及其形成机制

Bacterial community structure and assembly mechanisms in Sansha Bay,Fujian

细菌群落在水生生态系统中起着非常关键的作用。基于DNA和RNA高通量测序技术研究了福建三沙湾海域细菌的群落结构及其形成机制。结果发现:(1)三沙湾海域中共检测到细菌1476个操作分类单元(OTUs),其中γ-变形菌、α-变形菌、蓝细菌和拟杆菌为多样性最高的类群;(2)基于DNA和RNA高通量测序技术均发现这4种类群同时也是该海域细菌群落中的优势类群,但其代谢活性处于不同的状态,主要受到盐度、总氮、亚硝氮和无机磷酸盐浓度的调控;(3)三沙湾细菌群落结构在空间尺度上的分布存在差异,表现为地理位置上越相近的海区其细菌群落结构越相似。中性模型进一步分析发现,三沙湾海域细菌群落的形成主要受到中性过程的调控。本研究结果可为深入理解福建三沙湾海域中细菌群落结构及其形成机制提供理论依据。

Bacteria play an important role in the aquatic ecosystem.In our work,bacterial community structure and assembly mechanisms were studied in Sansha Bay in Fujian Province based on DNA and RNA high-throughput sequencing.Our results revealed that:(1) A total of 1 476 operational taxonomic units(OTUs) were detected,and γ-proteobacteria,α-proteobacteria,cyanobacteriia and bacteroidia were the most diverse bacterial groups.(2) γ-proteobacteria,α-proteobacteria,cyanobacteriia and bacteroidia were also the most abundant groups in Sansha Bay both in the DNA and RNA high-throughput sequencing results.The metabolic activities of these four dominant groups were different,and their metabolic activities were mainly regulated by salinity,total nitrogen,nitrite nitrogen and inorganic phosphate concentrations.(3) The bacterial community structure in Sansha Bay was different in spatial scale,and the closer the geographical location was,the more similar the bacterial community structure was.Neutral process was the main assembly mechanism affecting the construction of bacterial community in Sansha Bay.Our results were useful for the understanding the bacterial community structure and assembly mechanism in Sansha Bay,Fujian.