VGLL4基因多态性与幽门螺杆菌感染的关系

Association analysis between SNPs in VGLL4 gene and Helicobacter pylori infection

目的探讨残留样蛋白质家族成员4(VGLL4)基因单核苷酸多态性(SNP)与幽门螺杆菌(H.pylori)感染的关系。方法选取450例体检者的血液样本,采用ELISA法将样本分为幽门螺杆菌阴性组(n=220)和幽门螺杆菌阳性组(n=230)。采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术对VGLL4 SNP rs1803489、rs7617620、rs13078528位点进行基因分型。结果内蒙古包头地区汉族人群中VGLL4基因SNP rs1803489、rs7617620、rs13078528与幽门螺杆菌感染没有关联。结论在内蒙古包头地区的汉族人群中VGLL4基因SNP rs1803489、rs7617620、rs13078528在幽门螺杆菌感染中不起主要作用。

Objective To investigate the associations between the single nucleotide polymorphism(SNP)in vestigial like family member 4(VGLL4)gene and Helicobacter pylori(H.pylori)infection.Methods The blood samples of 450 normal physical examiners were collected,and the samples were divided into H.pylori negative group(n=220)and H.pylori positive group(n=230)using enzyme-linked immunosorbent assay(ELISA).SNP rs1803489,rs7617620,and rs13078528 in VGLL4 gene were genotyped using polymerase chain reaction(PCR)-restriction fragment length polymorphism(RFLP)technology.Results SNP rs1803489,rs7617620,and rs13078528 in VGLL4 gene were not associated with H.pylori infection in the Han population in Baotou,Inner Mongolia.Conclusion SNP rs1803489,rs7617620,and rs13078528 in VGLL4 gene may not play a major role in H.pylori infection in Baotou Han population.