牡荆素调控人结直肠癌细胞对奥沙利铂的敏感性

The Mechanism of Vitexin Regulating the Sensitivity of Human Colorectal Cancer Cells to Oxaliplatin

目的 探讨牡荆素对结直肠癌细胞化疗药物敏感性的影响。方法 使用CCK8实验检测牡荆素对人结直肠癌细胞HT29细胞活力的影响。Western blot实验检测牡荆素与奥沙利铂联合处理后,耐药相关蛋白P-gp,凋亡相关蛋白Bax,Bcl-2的表达变化。流式细胞术检测牡荆素和奥沙利铂联合用药后,对HT29细胞周期和细胞凋亡的影响。结果 CCK8实验显示,在浓度分别为30μmol,60μmol,90μmol及120μmol的牡荆素处理下,HT29细胞活力下降。Western blot显示,牡荆素和奥沙利铂联合用药组与单用奥沙利铂组比较,P-gp表达下调,Bax表达上调,Bcl-2表达下调;流式细胞术结果显示,联合用药组较单用奥沙利铂处理组细胞周期G1期延长,细胞凋亡水平差异具有统计学意义(P<0.05)。结论 牡荆素联合奥沙利铂可以促进人结直肠细胞凋亡,从而增强结直肠细胞对化疗药物奥沙利铂的敏感性。

Objective This study is aimed to verify whether vitexin can increase the sensitivity of colorectal cancer cells to chemotherapeutic drugs. Methods The cell-counting-kit-8 was applied to test the cell viability of HT29 cells. After HT29cells being co-treated with vitexin and oxaliplatin, western blot was used to check the expression of P-gp, Bax, and Bcl-2 in these groups. The flowcytometry was employed to test the apoptosis rate and cell cycle of HT29 cells after being cotreated with vitexin and oxaliplatin. Results The viability of HT29 cells was decreased gradually via treated with vitexin at the concentration gradients of 30μmol, 60μmol, 90μmol and 120μmol. Western blot showed that compared to the single drug treated group, the relative expression of P-gp and BCL-2 was declined, the expression of Bax was increased in cotreated group. The flow cycometry showed that the G1 phase was significantly expanded and the apoptosis rate of Co-treated group was increased compared to the oxaliplatin single treated group(P<0.05). Conclusion The combination use of vitexin and oxaliplatin can increase the apoptosis of HT29 cells and further increase its sensitivity to the chemotherapeutic drug oxaliplatin.