牛乳铁蛋白对热射病大鼠肝损害的保护作用

Protective Effect of Bovine Lactoferrin on Acute Liver Injury in Rats with Heat Stroke

目的研究预口服牛乳铁蛋白(bovine lactoferrin,BLF)对于热射病(heat stroke,HS)大鼠急性肝损伤的保护作用。方法64只大鼠按照完全随机化分组方式分为对照组(n=16)、热射病组(HS组,n=16)、低剂量干预组(LBLF组,n=16)和高剂量干预组(HBLF组,n=16)。对照组及HS组大鼠以磷酸盐缓冲液灌胃1周,LBLF组及HBLF组分别以100、200mg/kg BLF灌胃1周后建立热射病大鼠模型,排除死亡大鼠后,最终每组各8只大鼠用于样本的收集。获取大鼠的血清测定丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartateamino transferase,AST)、乳酸脱氢酶(lactate dehydrogenase,LDH)、超氧化物岐化酶(superoxide dismutase,SOD)活性及丙二醛(malondialdehyde,MDA)浓度。获取肝脏组织制作病理组织切片,比较各组大鼠病理切片的病理评分。结果与HS组比较,LBLF、HBLF组其核心体温到达HS发病标准(42℃)的平均时间均显著延长(P<0.001),HBLF组显著改善HS大鼠ALT、AST、LDH指标(P<0.05),HBLF组大鼠和HS组大鼠比较,SOD活性显著提高(P<0.001),MDA含量显著下降(P<0.001)。结论口服BLF可以提高大鼠热耐力,缓解HS大鼠肝脏脂质过氧化水平,减轻肝损伤。

Objective To study the protective effect of oral bovine lactoferrin(BLF)on acute liver injury in rats with heat stroke(HS).Methods Sixty-four rats were randomly divided into control group(n=16),HS group(n=16),low-dose BLF intervention(LBLF)group(n=16),high-dose BLF intervention(HBLF)group(n=16)using random number table.Control group and HS group were intragastrically administrated with phosphate buffer for 1 week,while LBLF group and HBLF group were intragastrically administrated with 100 and 200mg/kg BLF for 1 week,respectively,to establish a heat emission disease rat model.After excluding dead rats,8 rats in each group were finally collected for sample collection.Serum activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST),lactate dehydrogenase(LDH),superoxide dismutase(SOD)and malondialdehyde(MDA)were measured.Liver tissue and pathological tissue sections were obtained,and the pathological scores of each group were compared.Compared with HS group,the average time for core body temperature to reach the onset standard of HS(42℃)in LBLF and HBLF groups was significantly extended(P<0.001),and the indexes of ALT,AST and LDH of HS rats in HBLF group were significantly improved(P<0.05).SOD activity was significantly increased(P<0.001),MDA content was significantly decreased(P<0.001)Results The intervention group could delay the onset of HS compared with HS group.Meanwhile,biochemical indexes and oxidative stress related indexes were improved in HS rats.Conclusion BLF oral can improve the thermal endurance of rats,alleviate the level of lipid peroxidation and liver injury in HS rats.