UHRF1在口腔黏膜鳞癌中的表达及其对临床预后的价值研究

Expression of UHRF1 in oral squamous cell carcinoma and its value in clinical prognosis

目的 分析泛素样含PHD和环指域1(ubiquitin like with PHD and ring finger domains 1,UHRF1)在口腔黏膜鳞状细胞癌中的表达及其临床意义。方法 收集2008—2016年间,于中国科学技术大学附属第一医院口腔颌面外科接受手术治疗的原发性口腔鳞癌患者,利用免疫荧光染色评估患者的肿瘤组织、癌旁正常组织及侵袭前沿中的UHRF1表达,了解其表达差异性。以免疫反应强度分布指数(immunoreactivity intensity distribution index,IRIDI)值4分为界限,将患者分为UHRF1高表达组和低表达组,分析UHRF1表达水平与患者临床病理特征之间的关系,利用Kaplan-Meier法对肿瘤组织中UHRF1表达水平与患者生存率的相关性进行分析,运用Cox风险模型对影响口腔癌患者生存率的相关危险因素进行分析。结果 与肿瘤侵袭前沿、癌旁正常组织相比,UHRF1在口腔鳞癌肿瘤组织中高表达(P<0.01)。口腔鳞癌组织中UHRF1的表达水平与患者性别、肿瘤大小、浸润深度相关(P<0.05)。UHRF1低表达组和高表达组患者肿瘤复发、颈淋巴结转移、隐匿性淋巴结转移及生存率比较,均存在明显差异(P<0.05)。Cox风险回归模型结果显示,UHRF1的表达水平是影响口腔鳞癌患者生存率的危险因素(P=0.002)。结论 UHRF1在口腔鳞癌肿瘤组织中高表达,其表达水平与患者的临床预后密切相关,可作为口腔鳞癌有价值的预后指标。

Objective To analyze the expression and clinical significance of ubiquitin like with PHD and ring finger domain 1(UHRF1)in oral squamous cell carcinoma.Methods Patients with primary oral squamous cell carcinoma who underwent surgery at the department of oral and maxillofacial surgery,The First Affiliated Hospital of USTC from 2008 to 2016 were collected.The expression of UHRF1 in tumor tissues,adjacent normal tissues and invasion front were evaluated by immunofluorescence staining to explore the difference in UHRF1 expression.The patients were divided into high expression group and low expression group according to the value of immunoreactivity intensity distribution index(IRIDI).Relationship between the expression level of UHRF1 and clinicopathological characteristics of patients was analyzed.The correlation between the relative expression of UHRF1 in oral squamous cell carcinoma and survival rate was analyzed by Kaplan Meier method,and the relevant risk factors affecting the survival rate of patients with oral squamous cell carcinoma were analyzed by Cox risk regression model.Results Compared with the tumor invasion front and adjacent normal tissues,UHRF1 was highly expressed in oral squamous cell carcinoma(P<0.01).The expression level of UHRF1 in oral squamous cell carcinoma was correlated with gender,tumor size and depth of invasion(P<0.05).There were significant differences in tumor recurrence,cervical lymph node metastasis,occult lymph node metastasis and survival rate between low expression group and high expression group(P<0.05).Cox risk regression model showed that the expression level of UHRF1 was a risk factor for the prognosis of patients with oral squamous cell carcinoma(P=0.002).Conclusion UHRF1 is highly expressed in oral squamous cell carcinoma,and its expression level is closely related to the clinical prognosis of patients with oral squamous cell carcinoma.It can be used as a valuable prognostic index of oral squamous cell carcinoma.